Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   IRJ26_RS16030 Genome accession   NZ_CP064094
Coordinates   3044981..3045598 (+) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain N1108-5at     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3039981..3050598
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ26_RS15995 aroE 3040639..3041481 (+) 843 WP_003229967.1 shikimate dehydrogenase -
  IRJ26_RS16000 yhbY 3041475..3041765 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  IRJ26_RS16005 nadD 3041777..3042346 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  IRJ26_RS16010 yqeK 3042336..3042896 (+) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  IRJ26_RS16015 rsfS 3042914..3043270 (+) 357 WP_003229971.1 ribosome silencing factor -
  IRJ26_RS16020 yqeM 3043267..3044010 (+) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  IRJ26_RS16025 comER 3044076..3044897 (-) 822 WP_004398597.1 late competence protein ComER -
  IRJ26_RS16030 comEA 3044981..3045598 (+) 618 WP_004398514.1 competence protein ComEA Machinery gene
  IRJ26_RS16035 comEB 3045665..3046234 (+) 570 WP_003229978.1 ComE operon protein 2 -
  IRJ26_RS16040 comEC 3046238..3048568 (+) 2331 WP_009967776.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IRJ26_RS16045 yqzM 3048608..3048742 (-) 135 WP_003229983.1 YqzM family protein -
  IRJ26_RS16050 - 3048783..3048932 (+) 150 WP_003229985.1 hypothetical protein -
  IRJ26_RS16055 holA 3048972..3050015 (+) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  IRJ26_RS16060 rpsT 3050030..3050296 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=499330 IRJ26_RS16030 WP_004398514.1 3044981..3045598(+) (comEA) [Bacillus subtilis strain N1108-5at]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=499330 IRJ26_RS16030 WP_004398514.1 3044981..3045598(+) (comEA) [Bacillus subtilis strain N1108-5at]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39