Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H9K44_RS01650 Genome accession   NZ_CP064035
Coordinates   351814..352407 (+) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain DLM1799     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 346814..357407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H9K44_RS01635 (H9K44_01640) - 347530..350100 (-) 2571 WP_140268153.1 PBP1A family penicillin-binding protein -
  H9K44_RS01640 (H9K44_01645) pilM 350243..351259 (+) 1017 WP_017449463.1 type IV pilus assembly protein PilM Machinery gene
  H9K44_RS01645 (H9K44_01650) pilN 351243..351821 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  H9K44_RS01650 (H9K44_01655) pilO 351814..352407 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  H9K44_RS01655 (H9K44_01660) pilP 352397..352912 (+) 516 WP_025525190.1 pilus assembly protein PilP Machinery gene
  H9K44_RS01660 (H9K44_01665) pilQ 352944..354692 (+) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  H9K44_RS01665 (H9K44_01670) aroK 354881..355399 (+) 519 WP_005381319.1 shikimate kinase AroK -
  H9K44_RS01670 (H9K44_01675) aroB 355431..356531 (+) 1101 WP_005480443.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=498588 H9K44_RS01650 WP_005480482.1 351814..352407(+) (pilO) [Vibrio parahaemolyticus strain DLM1799]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=498588 H9K44_RS01650 WP_005480482.1 351814..352407(+) (pilO) [Vibrio parahaemolyticus strain DLM1799]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTTACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGCATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACACTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726