Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   IM712_RS11700 Genome accession   NZ_CP063768
Coordinates   2312932..2313546 (+) Length   204 a.a.
NCBI ID   WP_013352942.1    Uniprot ID   A0A411A874
Organism   Bacillus velezensis strain KMU01     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2307932..2318546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IM712_RS11665 aroE 2308619..2309461 (+) 843 WP_015417858.1 shikimate dehydrogenase -
  IM712_RS11670 yhbY 2309455..2309745 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  IM712_RS11675 - 2309756..2310325 (+) 570 WP_003152860.1 nicotinate-nucleotide adenylyltransferase -
  IM712_RS11680 yqeK 2310315..2310875 (+) 561 WP_077722612.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  IM712_RS11685 rsfS 2310893..2311249 (+) 357 WP_003152864.1 ribosome silencing factor -
  IM712_RS11690 - 2311246..2311983 (+) 738 WP_031378935.1 class I SAM-dependent DNA methyltransferase -
  IM712_RS11695 comER 2312052..2312873 (-) 822 WP_014305449.1 late competence protein ComER -
  IM712_RS11700 comEA 2312932..2313546 (+) 615 WP_013352942.1 helix-hairpin-helix domain-containing protein Machinery gene
  IM712_RS11705 - 2313613..2314182 (+) 570 WP_003152868.1 ComE operon protein 2 -
  IM712_RS11710 comEC 2314183..2316534 (+) 2352 WP_077722611.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IM712_RS11715 - 2316553..2316687 (-) 135 WP_003152870.1 YqzM family protein -
  IM712_RS11720 - 2316728..2316882 (+) 155 Protein_2262 hypothetical protein -
  IM712_RS11725 holA 2316922..2317963 (+) 1042 Protein_2263 DNA polymerase III subunit delta -
  IM712_RS11730 rpsT 2317980..2318246 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22190.27 Da        Isoelectric Point: 5.4841

>NTDB_id=496606 IM712_RS11700 WP_013352942.1 2312932..2313546(+) (comEA) [Bacillus velezensis strain KMU01]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=496606 IM712_RS11700 WP_013352942.1 2312932..2313546(+) (comEA) [Bacillus velezensis strain KMU01]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363