Detailed information NTG0496302

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BACVE_RS16260
• Genome accession: NZ_CP063687
• Coordinates: 3190327..3190941 (+)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain NST6
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3185327..3195941

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BACVE_RS16225 (BACVE_003274)	aroE	3186014..3186856 (+)	843	WP_017418183.1	shikimate dehydrogenase	-
BACVE_RS16230 (BACVE_003275)	yhbY	3186850..3187140 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BACVE_RS16235 (BACVE_003276)	-	3187151..3187720 (+)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
BACVE_RS16240 (BACVE_003277)	yqeK	3187710..3188291 (+)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BACVE_RS16245 (BACVE_003278)	rsfS	3188288..3188644 (+)	357	WP_003152864.1	ribosome silencing factor	-
BACVE_RS16250 (BACVE_003279)	-	3188641..3189378 (+)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
BACVE_RS16255 (BACVE_003280)	comER	3189447..3190268 (-)	822	WP_014305449.1	late competence protein ComER	-
BACVE_RS16260 (BACVE_003281)	comEA	3190327..3190941 (+)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
BACVE_RS16265 (BACVE_003282)	-	3191008..3191577 (+)	570	WP_003152868.1	ComE operon protein 2	-
BACVE_RS16270 (BACVE_003283)	comEC	3191578..3193929 (+)	2352	WP_025649830.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BACVE_RS16275	-	3193948..3194082 (-)	135	WP_003152870.1	YqzM family protein	-
BACVE_RS16280	-	3194123..3194274 (+)	152	Protein_3164	hypothetical protein	-
BACVE_RS16285 (BACVE_003284)	holA	3194314..3195409 (+)	1096	Protein_3165	DNA polymerase III subunit delta	-
BACVE_RS16290 (BACVE_003285)	rpsT	3195372..3195638 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=496302 BACVE_RS16260 WP_013352942.1 3190327..3190941(+) (comEA) [Bacillus velezensis strain NST6]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=496302 BACVE_RS16260 WP_013352942.1 3190327..3190941(+) (comEA) [Bacillus velezensis strain NST6]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363