Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   INP70_RS01300 Genome accession   NZ_CP063420
Coordinates   280233..280817 (+) Length   194 a.a.
NCBI ID   WP_000902746.1    Uniprot ID   -
Organism   Vibrio cholerae strain VC_hf7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 275233..285817
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP70_RS01285 oxyR 275638..276531 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  INP70_RS01290 - 276607..279084 (-) 2478 WP_000668743.1 penicillin-binding protein 1A -
  INP70_RS01295 pilM 279227..280243 (+) 1017 WP_000520445.1 type IV pilus assembly protein PilM Machinery gene
  INP70_RS01300 pilN 280233..280817 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  INP70_RS01305 pilO 280810..281400 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  INP70_RS01310 pilP 281390..281908 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  INP70_RS01315 pilQ 281934..283649 (+) 1716 WP_193836547.1 type IV pilus secretin PilQ Machinery gene
  INP70_RS01320 aroK 283836..284360 (+) 525 WP_000818616.1 shikimate kinase AroK -
  INP70_RS01325 aroB 284388..285473 (+) 1086 WP_000439805.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22022.45 Da        Isoelectric Point: 9.7294

>NTDB_id=495510 INP70_RS01300 WP_000902746.1 280233..280817(+) (pilN) [Vibrio cholerae strain VC_hf7]
MLHKVNLLPWRDARREAHKRRFLGLVTLGVLLAVLMQFAAGEYLGGQMALQQERIGYLQQHIFSLDQQIAKLKIAEEEHK
ALLTRLTVVEQLQQKRNKTTEFMNQMPNLIPEGVYVDKIKMNGHQIEITGISDSTARLATMLDNLEKSDKLTDVEMHEIV
SGNKRFGKQFQSFKVSFQILTPASNPQEGGAHNG

Nucleotide


Download         Length: 585 bp        

>NTDB_id=495510 INP70_RS01300 WP_000902746.1 280233..280817(+) (pilN) [Vibrio cholerae strain VC_hf7]
ATGTTGCATAAGGTCAACTTACTGCCGTGGCGTGATGCACGGCGTGAAGCACATAAGCGGCGCTTTTTGGGGTTAGTGAC
ACTCGGTGTTTTGCTGGCGGTGTTGATGCAATTTGCCGCCGGTGAATACTTGGGCGGACAAATGGCTTTGCAGCAAGAGC
GGATTGGTTATTTGCAACAACATATCTTCTCGCTGGATCAGCAGATCGCTAAGTTGAAGATCGCTGAAGAAGAACATAAG
GCGTTATTGACCCGTTTGACTGTGGTGGAGCAACTACAGCAAAAGCGCAATAAAACGACTGAGTTTATGAACCAAATGCC
CAACCTGATCCCTGAAGGCGTCTATGTCGACAAGATCAAGATGAATGGCCATCAGATAGAAATAACCGGGATTAGCGATT
CCACCGCTCGCTTGGCAACCATGCTCGATAACTTGGAGAAGTCGGACAAATTAACCGATGTTGAAATGCATGAAATCGTT
TCAGGGAATAAGCGATTTGGTAAGCAATTTCAGAGCTTTAAAGTCTCCTTCCAAATTTTAACCCCAGCCTCTAACCCGCA
AGAGGGAGGTGCACACAATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

99.485

100

0.995
  pilN Vibrio campbellii strain DS40M4

60.452

91.237

0.552