Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   INP70_RS01295 Genome accession   NZ_CP063420
Coordinates   279227..280243 (+) Length   338 a.a.
NCBI ID   WP_000520445.1    Uniprot ID   -
Organism   Vibrio cholerae strain VC_hf7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 274227..285243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP70_RS01280 - 274767..275498 (-) 732 WP_000495226.1 glutathione peroxidase -
  INP70_RS01285 oxyR 275638..276531 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  INP70_RS01290 - 276607..279084 (-) 2478 WP_000668743.1 penicillin-binding protein 1A -
  INP70_RS01295 pilM 279227..280243 (+) 1017 WP_000520445.1 type IV pilus assembly protein PilM Machinery gene
  INP70_RS01300 pilN 280233..280817 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  INP70_RS01305 pilO 280810..281400 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  INP70_RS01310 pilP 281390..281908 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  INP70_RS01315 pilQ 281934..283649 (+) 1716 WP_193836547.1 type IV pilus secretin PilQ Machinery gene
  INP70_RS01320 aroK 283836..284360 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37665.35 Da        Isoelectric Point: 7.1126

>NTDB_id=495509 INP70_RS01295 WP_000520445.1 279227..280243(+) (pilM) [Vibrio cholerae strain VC_hf7]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNNPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKHPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=495509 INP70_RS01295 WP_000520445.1 279227..280243(+) (pilM) [Vibrio cholerae strain VC_hf7]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATAATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTTTTTACTTCAGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGCAAACACCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.408

100

0.994
  pilM Vibrio campbellii strain DS40M4

48.225

100

0.482