Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KIM05_RS04665 Genome accession   NZ_CP074928
Coordinates   963206..963646 (-) Length   146 a.a.
NCBI ID   WP_000360902.1    Uniprot ID   A7ZHJ7
Organism   Escherichia coli strain 07_3636     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 958206..968646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM05_RS04640 (KIM05_04560) coaE 958696..959316 (-) 621 WP_001269520.1 dephospho-CoA kinase -
  KIM05_RS04645 (KIM05_04565) - 959341..959385 (+) 45 WP_120795372.1 protein YacM -
  KIM05_RS04650 (KIM05_04570) guaC 959541..960584 (+) 1044 WP_001217338.1 GMP reductase -
  KIM05_RS04655 (KIM05_04575) hofC 960619..961821 (-) 1203 WP_000157234.1 protein transport protein HofC -
  KIM05_RS04660 (KIM05_04580) pilB 961811..963196 (-) 1386 WP_001025155.1 type II secretion system protein GspE Machinery gene
  KIM05_RS04665 (KIM05_04585) pilA 963206..963646 (-) 441 WP_000360902.1 prepilin peptidase-dependent pilin Machinery gene
  KIM05_RS04670 (KIM05_04590) nadC 963849..964742 (-) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KIM05_RS04675 (KIM05_04595) ampD 964830..965381 (+) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KIM05_RS04680 (KIM05_04600) ampE 965378..966232 (+) 855 WP_000172006.1 beta-lactamase regulator AmpE -
  KIM05_RS04685 (KIM05_04605) aroP 966275..967648 (-) 1374 WP_012817732.1 aromatic amino acid transporter AroP -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15630.76 Da        Isoelectric Point: 4.5559

>NTDB_id=495512 KIM05_RS04665 WP_000360902.1 963206..963646(-) (pilA) [Escherichia coli strain 07_3636]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=495512 KIM05_RS04665 WP_000360902.1 963206..963646(-) (pilA) [Escherichia coli strain 07_3636]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCATC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZHJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

41.791

91.781

0.384
  pilA Haemophilus influenzae Rd KW20

41.045

91.781

0.377