Detailed information    

insolico Bioinformatically predicted

Overview

Name   ssbA   Type   Machinery gene
Locus tag   EKR40_RS12230 Genome accession   NZ_CP063381
Coordinates   2431578..2432057 (-) Length   159 a.a.
NCBI ID   WP_079400145.1    Uniprot ID   -
Organism   Listeria monocytogenes strain clinical isolate of L. monocytogenes isolate 12-05460     
Function   ssDNA binding (predicted from homology)   
DNA processing

Genomic Context

Location: 2426578..2437057
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EKR40_RS12185 (EKR40_12185) - 2426799..2428130 (-) 1332 WP_193470641.1 PBSX family phage terminase large subunit -
  EKR40_RS12190 (EKR40_12190) - 2428099..2428641 (-) 543 WP_068996252.1 terminase small subunit -
  EKR40_RS12195 (EKR40_12195) - 2428680..2428979 (-) 300 WP_061385639.1 hypothetical protein -
  EKR40_RS12200 (EKR40_12200) - 2429077..2429637 (-) 561 WP_238929053.1 hypothetical protein -
  EKR40_RS12205 (EKR40_12205) - 2429895..2430329 (-) 435 WP_003735131.1 ArpU family phage packaging/lysis transcriptional regulator -
  EKR40_RS12210 (EKR40_12210) - 2430348..2430512 (-) 165 WP_003727776.1 hypothetical protein -
  EKR40_RS12215 (EKR40_12215) - 2430641..2431024 (-) 384 WP_068996254.1 DUF2481 family protein -
  EKR40_RS12220 (EKR40_12220) - 2431028..2431432 (-) 405 WP_068996255.1 DUF1064 domain-containing protein -
  EKR40_RS12225 (EKR40_12225) - 2431377..2431559 (-) 183 WP_068996256.1 hypothetical protein -
  EKR40_RS12230 (EKR40_12230) ssbA 2431578..2432057 (-) 480 WP_079400145.1 single-stranded DNA-binding protein Machinery gene
  EKR40_RS12235 (EKR40_12235) - 2432057..2432458 (-) 402 WP_068996257.1 hypothetical protein -
  EKR40_RS12240 (EKR40_12240) - 2432455..2432640 (-) 186 WP_068996258.1 hypothetical protein -
  EKR40_RS12245 (EKR40_12245) - 2432642..2432797 (-) 156 WP_172425493.1 hypothetical protein -
  EKR40_RS12250 (EKR40_12250) - 2432824..2433033 (-) 210 WP_031645562.1 hypothetical protein -
  EKR40_RS12255 (EKR40_12255) - 2433030..2433245 (-) 216 WP_031645762.1 hypothetical protein -
  EKR40_RS12260 (EKR40_12260) - 2433349..2433909 (-) 561 WP_068996259.1 pentapeptide repeat-containing protein -
  EKR40_RS12265 (EKR40_12265) - 2433968..2434528 (-) 561 WP_014601511.1 DUF1642 domain-containing protein -
  EKR40_RS12270 (EKR40_12270) - 2434525..2434989 (-) 465 WP_039188046.1 class I SAM-dependent methyltransferase -
  EKR40_RS12275 (EKR40_12275) - 2434986..2435909 (-) 924 WP_119879452.1 DnaD domain-containing protein -
  EKR40_RS12280 (EKR40_12280) - 2435923..2436561 (-) 639 WP_009930473.1 ERF family protein -
  EKR40_RS12285 (EKR40_12285) - 2436566..2437042 (-) 477 WP_031644572.1 siphovirus Gp157 family protein -

Sequence

Protein


Download         Length: 159 a.a.        Molecular weight: 17665.42 Da        Isoelectric Point: 5.0012

>NTDB_id=494917 EKR40_RS12230 WP_079400145.1 2431578..2432057(-) (ssbA) [Listeria monocytogenes strain clinical isolate of L. monocytogenes isolate 12-05460]
MMNRVVLVGRLTKDPDLRYTPAGAAVATFTLAVNRTFTNQQGEREADFINCVVWRKPAENVANFLKKGSMAGVDGRVQTR
NYEDSDGKRVFVTEVVAESVQFLEPRNHAEGATSNNYQSEANYSNNNKTSSYRADTSQKSDSFANEGKPIDISDDDLPF

Nucleotide


Download         Length: 480 bp        

>NTDB_id=494917 EKR40_RS12230 WP_079400145.1 2431578..2432057(-) (ssbA) [Listeria monocytogenes strain clinical isolate of L. monocytogenes isolate 12-05460]
ATGATGAATCGTGTAGTGCTTGTAGGACGATTAACGAAAGATCCTGATTTACGATATACGCCAGCTGGTGCAGCAGTTGC
GACTTTTACATTAGCAGTAAATCGCACTTTCACTAACCAACAAGGAGAACGAGAAGCTGACTTTATTAATTGTGTTGTTT
GGCGTAAACCAGCGGAAAACGTTGCTAATTTCTTGAAGAAAGGAAGTATGGCGGGCGTTGATGGACGTGTTCAAACTCGT
AATTATGAGGATAGCGACGGTAAACGCGTTTTCGTTACAGAAGTAGTAGCTGAATCAGTTCAATTCTTAGAACCCAGAAA
CCACGCAGAAGGCGCTACATCGAATAATTATCAAAGCGAGGCTAATTATTCAAATAACAATAAAACAAGTTCATATCGAG
CGGATACGAGCCAGAAGAGTGATTCATTTGCGAATGAGGGTAAGCCGATAGATATTTCAGATGATGATTTGCCATTTTGA

Domains

Predicted by InterproScan.

Single-strand binding protein family (SSB)

(2-103)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

66.86

100

0.723
  ssb Latilactobacillus sakei subsp. sakei 23K

57.059

100

0.61
  ssbB Bacillus subtilis subsp. subtilis str. 168

60.714

70.44

0.428