Detailed information    

insolico Bioinformatically predicted

Overview

Name   uvrC   Type   Machinery gene
Locus tag   KIM25_RS10840 Genome accession   NZ_CP074856
Coordinates   2188212..2190044 (+) Length   610 a.a.
NCBI ID   WP_001283424.1    Uniprot ID   B1IZZ1
Organism   Escherichia coli strain 2013C_3997_C1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2183212..2195044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM25_RS10800 (KIM25_10745) dcyD 2183438..2184424 (+) 987 WP_001128238.1 D-cysteine desulfhydrase -
  KIM25_RS10805 (KIM25_10750) tcyL 2184439..2185107 (+) 669 WP_001158220.1 cystine ABC transporter permease -
  KIM25_RS10810 (KIM25_10755) tcyN 2185104..2185856 (+) 753 WP_001272994.1 L-cystine ABC transporter ATP-binding protein TcyN -
  KIM25_RS10815 (KIM25_10760) sdiA 2186086..2186808 (+) 723 WP_001154265.1 transcriptional regulator SdiA -
  KIM25_RS10820 (KIM25_10765) yecF 2186876..2187100 (-) 225 WP_000106474.1 DUF2594 family protein YecF -
  KIM25_RS10825 (KIM25_10770) yecU 2187087..2187263 (-) 177 WP_001342215.1 protein YecU -
  KIM25_RS10830 - 2187301..2187417 (-) 117 WP_001302039.1 hypothetical protein -
  KIM25_RS10835 (KIM25_10775) letA 2187559..2188215 (+) 657 WP_000611328.1 UvrY/SirA/GacA family response regulator transcription factor Regulator
  KIM25_RS10840 (KIM25_10780) uvrC 2188212..2190044 (+) 1833 WP_001283424.1 excinuclease ABC subunit UvrC Machinery gene
  KIM25_RS10845 (KIM25_10785) pgsA 2190101..2190649 (+) 549 WP_001160187.1 CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase -
  KIM25_RS10865 (KIM25_10805) yecA 2191299..2191964 (+) 666 WP_000847902.1 UPF0149 family protein YecA -
  KIM25_RS10870 (KIM25_10810) tyrP 2192026..2193237 (-) 1212 WP_000797573.1 tyrosine transporter TyrP -
  KIM25_RS10875 (KIM25_10815) yecH 2193428..2193667 (+) 240 WP_000377224.1 YecH family metal-binding protein -
  KIM25_RS10880 (KIM25_10820) ftnA 2193705..2194202 (-) 498 WP_000917208.1 non-heme ferritin -
  KIM25_RS10885 (KIM25_10825) yecR 2194373..2194696 (-) 324 WP_001237869.1 YecR-like lipofamily protein -

Sequence

Protein


Download         Length: 610 a.a.        Molecular weight: 68216.09 Da        Isoelectric Point: 9.3685

>NTDB_id=493864 KIM25_RS10840 WP_001283424.1 2188212..2190044(+) (uvrC) [Escherichia coli strain 2013C_3997_C1]
MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLSSYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLL
EHNYIKLYQPRYNVLLRDDKSYPFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETASQNLEFEEAARIRDQIQAVRRVT
EKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKVLGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLD
FNLSDKTLLADSLSELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEVKRMECFDI
SHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRYGKAIDDSKIPDVILIDGGKGQLAQAKNVFA
ELDVSWDKNHPLLLGVAKGVDRKAGLETLFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLET
IEGVGPKRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH

Nucleotide


Download         Length: 1833 bp        

>NTDB_id=493864 KIM25_RS10840 WP_001283424.1 2188212..2190044(+) (uvrC) [Escherichia coli strain 2013C_3997_C1]
GTGAGTGATCAGTTTGACGCAAAAGCGTTTTTAAAAACCGTAACCAGCCAGCCAGGCGTTTATCGCATGTACGATGCTGG
TGGTACGGTTATCTATGTCGGCAAAGCGAAAGACCTGAAAAAACGGCTTTCCAGCTATTTCCGTAGCAACCTCGCTTCGC
GCAAAACCGAAGCGCTGGTCGCCCAGATCCAGCAAATTGATGTAACGGTTACTCATACAGAAACCGAAGCGCTGTTGCTG
GAACACAACTACATCAAACTCTATCAGCCGCGTTACAACGTTTTGCTACGCGATGATAAATCTTATCCTTTTATCTTCCT
GAGTGGCGATACCCACCCGCGTCTGGCGATGCATCGTGGTGCGAAGCATGCCAAAGGTGAATATTTCGGCCCGTTCCCGA
ATGGCTATGCCGTACGTGAAACACTGGCGCTACTGCAAAAGATTTTCCCCATTCGCCAGTGCGAAAATAGTGTTTATCGC
AATCGCTCGCGTCCGTGTCTGCAATACCAGATAGGGCGCTGTCTGGGACCGTGCGTTGAAGGACTGGTGAGTGAAGAAGA
ATACGCTCAGCAGGTCGAGTATGTGCGCCTGTTTTTGTCTGGCAAAGATGATCAGGTGCTTACGCAACTCATTAGTCGTA
TGGAAACTGCCAGCCAGAATCTGGAGTTTGAAGAAGCTGCACGTATTCGCGACCAAATTCAGGCGGTGCGACGCGTCACC
GAAAAACAATTCGTTTCCAATACCGGCGACGACCTCGACGTTATTGGTGTGGCGTTCGATGCGGGCATGGCTTGTGTCCA
CGTATTGTTCATTCGTCAGGGCAAAGTGCTCGGCAGCCGCAGCTATTTCCCGAAAGTGCCTGGCGGTACGGAACTGAGCG
AGGTGGTAGAAACCTTCGTAGGCCAGTTCTATTTACAAGGCAGCCAGATGCGCACCTTACCGGGTGAGATCCTGCTCGAT
TTTAATCTTAGCGATAAAACGCTGCTCGCCGATTCCCTTTCAGAACTGGCGGGACGCAAGATTAATGTTCAAACCAAACC
TCGCGGCGATAGGGCGCGTTATCTGAAACTCGCGCGCACCAATGCGGCGACGGCCTTAACCAGCAAACTTTCGCAGCAAT
CTACCGTTCACCAGCGACTGACCGCGCTTGCCAGCGTGTTGAAATTGCCGGAAGTGAAGCGGATGGAGTGCTTTGACATC
AGCCATACCATGGGCGAACAAACCGTCGCTTCCTGTGTGGTGTTTGATGCTAACGGCCCGCTGCGTGCGGAGTATCGGCG
CTATAACATTACAGGCATCACGCCGGGCGATGATTATGCGGCGATGAATCAGGTGCTGCGTCGGCGTTATGGTAAAGCCA
TTGACGACAGTAAGATCCCGGATGTGATCCTTATCGACGGCGGCAAAGGCCAGCTTGCGCAGGCGAAAAATGTCTTCGCC
GAACTGGATGTCTCATGGGATAAAAATCATCCACTGCTACTTGGCGTTGCCAAAGGAGTAGATCGTAAGGCTGGGCTGGA
AACACTGTTCTTTGAGCCGGAAGGTGAGGGATTCAGTTTGCCGCCAGATTCTCCCGCGCTGCATGTTATCCAGCATATTC
GCGATGAATCACACGATCACGCGATTGGCGGGCACCGTAAAAAACGGGCGAAGGTCAAAAATACCAGCTCCCTGGAAACC
ATTGAAGGCGTCGGGCCAAAACGTCGGCAAATGTTGTTGAAATATATGGGCGGTTTGCAAGGTTTACGTAACGCCAGCGT
CGAGGAAATTGCAAAAGTGCCGGGTATTTCGCAAGGTCTGGCAGAAAAGATCTTCTGGTCGTTGAAACATTGA

Domains

Predicted by InterProScan.

UvrB/uvrC motif (UVR)

(205-237)

Helix-hairpin-helix domain (HHH_5)

(558-609)

GIY-YIG catalytic domain (GIY-YIG)

(18-93)

UvrC RNAse H endonuclease domain (UvrC_RNaseH_dom)

(385-542)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B1IZZ1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  uvrC Streptococcus pneumoniae TIGR4

37.891

99.508

0.377
  uvrC Streptococcus pneumoniae R6

37.562

99.508

0.374
  uvrC Streptococcus pneumoniae D39

37.562

99.508

0.374