Detailed information
Bioinformatically predicted
Overview
| Name | comEA | Type | Machinery gene |
| Locus tag | IMZ18_RS05305 | Genome accession | NZ_CP063151 |
| Coordinates | 996698..997315 (+) | Length | 205 a.a. |
| NCBI ID | WP_014480312.1 | Uniprot ID | - |
| Organism | Bacillus subtilis subsp. subtilis strain CMIN-4 | ||
| Function | dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 962148..1001732 | 996698..997315 | within | 0 |
Gene organization within MGE regions
Location: 962148..1001732
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| IMZ18_RS05070 (IMZ18_05070) | - | 962148..962328 (+) | 181 | Protein_991 | hypothetical protein | - |
| IMZ18_RS22415 | - | 962462..962692 (+) | 231 | WP_014480347.1 | hypothetical protein | - |
| IMZ18_RS05080 (IMZ18_05080) | - | 963011..963259 (+) | 249 | Protein_993 | hypothetical protein | - |
| IMZ18_RS05085 (IMZ18_05085) | - | 963222..963344 (+) | 123 | Protein_994 | RusA family crossover junction endodeoxyribonuclease | - |
| IMZ18_RS05090 (IMZ18_05090) | - | 963427..963579 (+) | 153 | WP_049832653.1 | XtrA/YqaO family protein | - |
| IMZ18_RS05095 (IMZ18_05095) | - | 963718..963984 (-) | 267 | WP_033881358.1 | hypothetical protein | - |
| IMZ18_RS05100 (IMZ18_05100) | - | 964601..965080 (+) | 480 | WP_014480344.1 | hypothetical protein | - |
| IMZ18_RS22420 | - | 965235..965300 (+) | 66 | Protein_998 | hypothetical protein | - |
| IMZ18_RS05105 (IMZ18_05105) | - | 965473..965778 (-) | 306 | WP_123772463.1 | hypothetical protein | - |
| IMZ18_RS05110 (IMZ18_05110) | terS | 965905..966470 (+) | 566 | Protein_1000 | phage terminase small subunit | - |
| IMZ18_RS05115 (IMZ18_05115) | - | 966468..966904 (+) | 437 | Protein_1001 | phage tail tube protein | - |
| IMZ18_RS05120 (IMZ18_05120) | - | 967191..967277 (-) | 87 | WP_072592549.1 | putative holin-like toxin | - |
| IMZ18_RS22425 | - | 967455..967552 (+) | 98 | Protein_1003 | N-acetylmuramoyl-L-alanine amidase | - |
| IMZ18_RS05130 (IMZ18_05130) | istB | 967870..968628 (-) | 759 | WP_014479891.1 | IS21-like element helper ATPase IstB | - |
| IMZ18_RS05135 (IMZ18_05135) | istA | 968625..970172 (-) | 1548 | WP_014480339.1 | IS21 family transposase | - |
| IMZ18_RS05140 (IMZ18_05140) | - | 971012..971302 (-) | 291 | WP_014480337.1 | contact-dependent growth inhibition system immunity protein | - |
| IMZ18_RS05145 (IMZ18_05145) | atxG | 971412..971989 (-) | 578 | Protein_1007 | suppressor of fused domain protein | - |
| IMZ18_RS05150 (IMZ18_05150) | - | 972257..972490 (-) | 234 | WP_224588641.1 | hypothetical protein | - |
| IMZ18_RS05155 (IMZ18_05155) | - | 972579..972782 (+) | 204 | WP_123772462.1 | hypothetical protein | - |
| IMZ18_RS05160 (IMZ18_05160) | - | 973090..973602 (-) | 513 | WP_014477426.1 | hypothetical protein | - |
| IMZ18_RS05165 (IMZ18_05165) | cdiI | 973674..974033 (-) | 360 | WP_014480334.1 | ribonuclease toxin immunity protein CdiI | - |
| IMZ18_RS05170 (IMZ18_05170) | - | 974130..974582 (-) | 453 | WP_014480333.1 | SMI1/KNR4 family protein | - |
| IMZ18_RS05175 (IMZ18_05175) | - | 974681..975121 (-) | 441 | WP_014480332.1 | SMI1/KNR4 family protein | - |
| IMZ18_RS05180 (IMZ18_05180) | - | 975524..975811 (-) | 288 | WP_014480331.1 | hypothetical protein | - |
| IMZ18_RS22755 (IMZ18_05185) | - | 975825..977751 (-) | 1927 | Protein_1015 | T7SS effector LXG polymorphic toxin | - |
| IMZ18_RS05190 (IMZ18_05190) | - | 977933..979060 (+) | 1128 | WP_014480328.1 | Rap family tetratricopeptide repeat protein | - |
| IMZ18_RS05195 (IMZ18_05195) | - | 979800..980195 (-) | 396 | WP_014480327.1 | VOC family protein | - |
| IMZ18_RS05200 (IMZ18_05200) | - | 981137..982027 (-) | 891 | WP_014480326.1 | LysR family transcriptional regulator | - |
| IMZ18_RS05205 (IMZ18_05205) | fumC | 982194..983582 (+) | 1389 | WP_014480325.1 | class II fumarate hydratase | - |
| IMZ18_RS22445 | - | 983801..984013 (+) | 213 | Protein_1020 | recombinase family protein | - |
| IMZ18_RS05215 (IMZ18_05215) | - | 984010..984108 (-) | 99 | WP_031600702.1 | hypothetical protein | - |
| IMZ18_RS05220 (IMZ18_05220) | sigK | 984108..984836 (-) | 729 | WP_013308023.1 | RNA polymerase sporulation sigma factor SigK | - |
| IMZ18_RS05225 (IMZ18_05225) | nucA/comI | 985032..985442 (+) | 411 | WP_009967785.1 | sporulation-specific Dnase NucB | Machinery gene |
| IMZ18_RS05230 (IMZ18_05230) | yqeB | 985475..986197 (-) | 723 | WP_014480321.1 | hypothetical protein | - |
| IMZ18_RS05235 (IMZ18_05235) | gnd | 986448..987341 (+) | 894 | WP_014480320.1 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| IMZ18_RS05240 (IMZ18_05240) | yqeD | 987360..987986 (-) | 627 | WP_014480319.1 | TVP38/TMEM64 family protein | - |
| IMZ18_RS05245 (IMZ18_05245) | cwlH | 988173..988925 (+) | 753 | WP_014480318.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| IMZ18_RS05250 (IMZ18_05250) | yqeF | 989177..989908 (+) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| IMZ18_RS05255 (IMZ18_05255) | - | 990214..990354 (-) | 141 | WP_003226124.1 | sporulation histidine kinase inhibitor Sda | - |
| IMZ18_RS05260 (IMZ18_05260) | yqeG | 990716..991234 (+) | 519 | WP_003226126.1 | YqeG family HAD IIIA-type phosphatase | - |
| IMZ18_RS05265 (IMZ18_05265) | yqeH | 991238..992338 (+) | 1101 | WP_003229966.1 | ribosome biogenesis GTPase YqeH | - |
| IMZ18_RS05270 (IMZ18_05270) | aroE | 992356..993198 (+) | 843 | WP_014480317.1 | shikimate dehydrogenase | - |
| IMZ18_RS05275 (IMZ18_05275) | yhbY | 993192..993482 (+) | 291 | WP_003226133.1 | ribosome assembly RNA-binding protein YhbY | - |
| IMZ18_RS05280 (IMZ18_05280) | nadD | 993494..994063 (+) | 570 | WP_004398676.1 | nicotinate-nucleotide adenylyltransferase | - |
| IMZ18_RS05285 (IMZ18_05285) | yqeK | 994053..994613 (+) | 561 | WP_014480316.1 | bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK | - |
| IMZ18_RS05290 (IMZ18_05290) | rsfS | 994631..994987 (+) | 357 | WP_014480315.1 | ribosome silencing factor | - |
| IMZ18_RS05295 (IMZ18_05295) | yqeM | 994984..995727 (+) | 744 | WP_014480314.1 | class I SAM-dependent methyltransferase | - |
| IMZ18_RS05300 (IMZ18_05300) | comER | 995793..996614 (-) | 822 | WP_014480313.1 | late competence protein ComER | - |
| IMZ18_RS05305 (IMZ18_05305) | comEA | 996698..997315 (+) | 618 | WP_014480312.1 | competence protein ComEA | Machinery gene |
| IMZ18_RS05310 (IMZ18_05310) | comEB | 997382..997951 (+) | 570 | WP_003229978.1 | ComE operon protein 2 | - |
| IMZ18_RS05315 (IMZ18_05315) | comEC | 997955..1000285 (+) | 2331 | WP_033881047.1 | DNA internalization-related competence protein ComEC/Rec2 | Machinery gene |
| IMZ18_RS05320 (IMZ18_05320) | yqzM | 1000325..1000459 (-) | 135 | WP_003229983.1 | YqzM family protein | - |
| IMZ18_RS05325 (IMZ18_05325) | - | 1000500..1000649 (+) | 150 | WP_003229985.1 | hypothetical protein | - |
| IMZ18_RS05330 (IMZ18_05330) | holA | 1000689..1001732 (+) | 1044 | WP_014480310.1 | DNA polymerase III subunit delta | - |
Sequence
Protein
Download Length: 205 a.a. Molecular weight: 21781.51 Da Isoelectric Point: 4.7220
>NTDB_id=493452 IMZ18_RS05305 WP_014480312.1 996698..997315(+) (comEA) [Bacillus subtilis subsp. subtilis strain CMIN-4]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEEIAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK
Nucleotide
Download Length: 618 bp
>NTDB_id=493452 IMZ18_RS05305 WP_014480312.1 996698..997315(+) (comEA) [Bacillus subtilis subsp. subtilis strain CMIN-4]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAATAGCAGTGCAGCAAGGTGGCGGAGGGTCAG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| comEA | Bacillus subtilis subsp. subtilis str. 168 |
99.512 |
100 |
0.995 |
| comEA | Staphylococcus aureus MW2 |
37.273 |
100 |
0.4 |
| comEA | Staphylococcus aureus N315 |
36.364 |
100 |
0.39 |