Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IMY14_RS13215 Genome accession   NZ_CP063150
Coordinates   2540315..2541385 (-) Length   356 a.a.
NCBI ID   WP_015483431.1    Uniprot ID   -
Organism   Bacillus subtilis strain s-16     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2535315..2546385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMY14_RS13170 tapA 2535795..2536556 (-) 762 WP_047182870.1 amyloid fiber anchoring/assembly protein TapA -
  IMY14_RS13175 yqzG 2536826..2537152 (+) 327 WP_047183570.1 YqzG/YhdC family protein -
  IMY14_RS13180 spoIITA 2537194..2537373 (-) 180 WP_003230176.1 YqzE family protein -
  IMY14_RS13185 comGG 2537445..2537819 (-) 375 WP_014480253.1 ComG operon protein ComGG Machinery gene
  IMY14_RS13190 comGF 2537820..2538203 (-) 384 WP_015384087.1 ComG operon protein ComGF Machinery gene
  IMY14_RS13195 comGE 2538229..2538576 (-) 348 WP_047182872.1 ComG operon protein 5 Machinery gene
  IMY14_RS13200 comGD 2538560..2538991 (-) 432 WP_015384089.1 comG operon protein ComGD Machinery gene
  IMY14_RS13205 comGC 2538981..2539277 (-) 297 WP_014477332.1 comG operon protein ComGC Machinery gene
  IMY14_RS13210 comGB 2539291..2540328 (-) 1038 WP_047182873.1 comG operon protein ComGB Machinery gene
  IMY14_RS13215 comGA 2540315..2541385 (-) 1071 WP_015483431.1 competence protein ComGA Machinery gene
  IMY14_RS13225 corA 2541795..2542748 (-) 954 WP_015483432.1 magnesium transporter CorA -
  IMY14_RS13230 yqhB 2542891..2544219 (+) 1329 WP_003230150.1 hemolysin family protein -
  IMY14_RS13235 rsbRD 2544272..2545108 (-) 837 WP_015384093.1 RsbT co-antagonist protein RsbRD -
  IMY14_RS13245 mgsR 2545332..2545712 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  IMY14_RS13250 yqgY 2545944..2546189 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=493406 IMY14_RS13215 WP_015483431.1 2540315..2541385(-) (comGA) [Bacillus subtilis strain s-16]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYITTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=493406 IMY14_RS13215 WP_015483431.1 2540315..2541385(-) (comGA) [Bacillus subtilis strain s-16]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGTGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCATTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGATCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTGACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTAGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATATAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997
  pilF Thermus thermophilus HB27

36.827

99.157

0.365