Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   KIM35_RS23485 Genome accession   NZ_CP074815
Coordinates   4625013..4625672 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain 7_33_80A     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4620013..4630672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KIM35_RS23465 (KIM35_23170) plsC 4620160..4620897 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  KIM35_RS23470 (KIM35_23175) parC 4621130..4623388 (-) 2259 WP_001281899.1 DNA topoisomerase IV subunit A -
  KIM35_RS23475 (KIM35_23180) ygiV 4623934..4624416 (-) 483 WP_000183500.1 AraC family transcriptional regulator -
  KIM35_RS23480 (KIM35_23185) ygiW 4624469..4624861 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  KIM35_RS23485 (KIM35_23190) ciaR 4625013..4625672 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  KIM35_RS23490 (KIM35_23195) qseC 4625669..4627018 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  KIM35_RS23495 (KIM35_23200) ygiZ 4627064..4627396 (-) 333 WP_000912120.1 DUF2645 family protein -
  KIM35_RS23500 (KIM35_23205) mdaB 4627715..4628296 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  KIM35_RS23505 (KIM35_23210) ygiN 4628327..4628641 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  KIM35_RS23510 (KIM35_23215) parE 4628689..4630581 (-) 1893 WP_032345138.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=493086 KIM35_RS23485 WP_001221502.1 4625013..4625672(+) (ciaR) [Escherichia coli strain 7_33_80A]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=493086 KIM35_RS23485 WP_001221502.1 4625013..4625672(+) (ciaR) [Escherichia coli strain 7_33_80A]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365