Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   INP94_RS00270 Genome accession   NZ_CP063120
Coordinates   57178..57855 (+) Length   225 a.a.
NCBI ID   WP_197543657.1    Uniprot ID   A0A7M1NWP4
Organism   Haemophilus parainfluenzae strain M1C137_2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 52178..62855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  INP94_RS00245 (INP94_00245) rppH 52335..52928 (-) 594 WP_049371640.1 RNA pyrophosphohydrolase -
  INP94_RS00250 (INP94_00250) ampD 53414..53977 (-) 564 WP_049371639.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  INP94_RS00255 (INP94_00255) pilA 54120..54563 (+) 444 WP_005697917.1 prepilin peptidase-dependent pilin Machinery gene
  INP94_RS00260 (INP94_00260) pilB 54570..55949 (+) 1380 WP_197543655.1 GspE/PulE family protein Machinery gene
  INP94_RS00265 (INP94_00265) pilC 55958..57181 (+) 1224 WP_197543656.1 type II secretion system F family protein Machinery gene
  INP94_RS00270 (INP94_00270) pilD 57178..57855 (+) 678 WP_197543657.1 prepilin peptidase Machinery gene
  INP94_RS00275 (INP94_00275) coaE 57944..58564 (+) 621 WP_197543658.1 dephospho-CoA kinase -
  INP94_RS00280 (INP94_00280) yacG 58557..58763 (+) 207 WP_005695505.1 DNA gyrase inhibitor YacG -
  INP94_RS00285 (INP94_00285) - 58760..59041 (+) 282 WP_197543659.1 GNAT family N-acetyltransferase -
  INP94_RS00290 (INP94_00290) rhlB 59132..60343 (+) 1212 WP_197543660.1 ATP-dependent RNA helicase RhlB -
  INP94_RS00295 (INP94_00295) mreD 60432..60920 (-) 489 WP_197543661.1 rod shape-determining protein MreD -
  INP94_RS00300 (INP94_00300) mreC 60920..61984 (-) 1065 WP_197543662.1 rod shape-determining protein MreC -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 25948.91 Da        Isoelectric Point: 7.3414

>NTDB_id=493065 INP94_RS00270 WP_197543657.1 57178..57855(+) (pilD) [Haemophilus parainfluenzae strain M1C137_2]
MMTFAFFLLGGLAGIFAWLYIVRFIPNLQQEIYQNYIELYPENRPIFHSEKAAIQSQKCGHIFLYFLGLGLCFAALYYFL
QDELFTLWLAITLSLIFVISWLDWHYQLISPTPCLFLFFLGLFGAHQKLSILTLSQSLESAVSFFGVFYIIYHLSKWFYK
KEALGRGDYWLALGIGAYLTIAHLPLFLFIACLLGIIGYWISRQPVLPFAPFLCLSLIITSAINL

Nucleotide


Download         Length: 678 bp        

>NTDB_id=493065 INP94_RS00270 WP_197543657.1 57178..57855(+) (pilD) [Haemophilus parainfluenzae strain M1C137_2]
ATGATGACATTCGCCTTTTTCTTATTGGGTGGTTTAGCGGGCATCTTTGCTTGGCTTTATATCGTTCGTTTTATTCCTAA
TCTCCAACAAGAAATTTATCAGAACTATATCGAACTGTATCCTGAAAACAGGCCCATTTTCCATAGCGAAAAAGCAGCGA
TTCAATCTCAAAAGTGCGGTCATATTTTCCTTTATTTTTTAGGGCTTGGACTCTGTTTTGCTGCACTCTATTATTTTCTT
CAGGATGAATTATTTACTTTATGGCTCGCGATTACGCTTAGTCTCATCTTTGTTATCAGTTGGCTAGACTGGCACTATCA
ACTGATTTCACCTACACCTTGCCTGTTTTTATTTTTCCTGGGTTTATTCGGCGCACATCAAAAACTTTCAATTCTGACGC
TTTCTCAAAGTTTAGAAAGTGCGGTTAGTTTTTTCGGTGTTTTTTATATCATCTATCATTTGTCTAAATGGTTTTATAAG
AAGGAAGCGTTAGGCCGTGGCGATTATTGGCTCGCTTTAGGAATAGGCGCTTATCTCACCATCGCACACTTACCACTTTT
CTTATTCATTGCCTGTCTATTAGGCATCATAGGTTATTGGATTTCTCGTCAGCCCGTTTTACCTTTCGCGCCCTTTCTTT
GCTTATCGTTAATCATCACCAGTGCAATCAACCTATAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7M1NWP4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae Rd KW20

52.489

98.222

0.516
  pilD Haemophilus influenzae 86-028NP

51.584

98.222

0.507