Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   TTJL18_RS04150 Genome accession   NC_017587
Coordinates   754908..755609 (-) Length   233 a.a.
NCBI ID   WP_014629427.1    Uniprot ID   H9ZQX5
Organism   Thermus thermophilus JL-18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 749908..760609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TTJL18_RS04125 (TtJL18_0827) - 750480..751037 (+) 558 WP_041444427.1 Uma2 family endonuclease -
  TTJL18_RS04130 (TtJL18_0829) - 751381..751893 (-) 513 WP_222842107.1 coiled-coil domain-containing protein -
  TTJL18_RS04135 (TtJL18_0830) - 752086..752445 (-) 360 WP_014629424.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TTJL18_RS04140 (TtJL18_0831) - 752518..752913 (-) 396 WP_014629425.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  TTJL18_RS04145 (TtJL18_0832) comZ 753179..754897 (-) 1719 WP_014629426.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  TTJL18_RS04150 (TtJL18_0833) pilA/pilA3 754908..755609 (-) 702 WP_014629427.1 PilW family protein Machinery gene
  TTJL18_RS04155 (TtJL18_0834) pilA/pilA2 755606..756187 (-) 582 WP_014629428.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  TTJL18_RS04160 (TtJL18_0835) - 756184..756657 (-) 474 WP_014629429.1 GspH/FimT family protein -
  TTJL18_RS04165 (TtJL18_0836) pilA/pilA1 756713..757183 (-) 471 WP_014629430.1 GspH/FimT family protein Machinery gene
  TTJL18_RS04170 (TtJL18_0837) - 757349..758329 (+) 981 WP_014629431.1 YpdA family putative bacillithiol disulfide reductase -
  TTJL18_RS04175 (TtJL18_0838) ilvB 758485..760173 (+) 1689 WP_014629432.1 biosynthetic-type acetolactate synthase large subunit -

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 24920.35 Da        Isoelectric Point: 7.4626

>NTDB_id=49122 TTJL18_RS04150 WP_014629427.1 754908..755609(-) (pilA/pilA3) [Thermus thermophilus JL-18]
MKRGFTLVEVLVAMAILVVVLAVGVRYFASTSELARNTQARSELQDRVRMVMQVVTADLQMAGARYWNSGNQNRAFSLPL
PPLSGSDGGPKDTLTLYYVTSLRDLASACRRVDYGFDGDTLRRSDLNATPSSGSDCTTPSSNLQPLAEGMLALDIQYQCS
DGSQKDTPDCGTDAYPRSAKVTVAGYSLTPVTNPGPASLTTVTGKTLACPQGRACYALTQEVLMPNLKPLPTP

Nucleotide


Download         Length: 702 bp        

>NTDB_id=49122 TTJL18_RS04150 WP_014629427.1 754908..755609(-) (pilA/pilA3) [Thermus thermophilus JL-18]
ATGAAGCGCGGGTTCACCCTGGTGGAAGTCCTGGTGGCCATGGCCATCTTGGTGGTGGTCCTGGCCGTGGGCGTGCGCTA
CTTCGCAAGCACAAGCGAGCTCGCCCGGAACACCCAGGCAAGGAGCGAGCTCCAGGACCGGGTGCGCATGGTGATGCAGG
TGGTGACCGCCGACCTGCAGATGGCGGGGGCCCGCTACTGGAATAGCGGCAACCAAAACCGGGCCTTCAGCCTCCCCCTT
CCCCCTCTTTCGGGAAGCGACGGAGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTCCGGGACCTGGCCTC
CGCCTGCCGCCGGGTGGACTACGGGTTTGACGGGGACACCCTACGCCGGAGCGACCTGAACGCCACCCCCTCTTCGGGAA
GCGATTGCACCACCCCCTCTTCGAACTTGCAGCCTTTGGCCGAAGGGATGTTGGCCCTGGACATCCAATACCAGTGCAGC
GACGGAAGCCAGAAGGACACCCCCGACTGCGGTACAGACGCCTACCCCCGCTCGGCCAAGGTCACGGTGGCGGGCTACTC
CCTGACCCCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCGGAAAGACCCTCGCCTGCCCCCAGGGCCGGG
CCTGCTACGCCCTCACCCAGGAGGTCCTCATGCCCAACCTCAAGCCCCTGCCCACCCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H9ZQX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

95.708

100

0.957

Multiple sequence alignment