Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   IHV08_RS13475 Genome accession   NZ_CP062497
Coordinates   2587498..2588115 (-) Length   205 a.a.
NCBI ID   WP_033884458.1    Uniprot ID   -
Organism   Bacillus subtilis strain CV16     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2582498..2593115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IHV08_RS13445 (IHV08_13445) rpsT 2582800..2583066 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  IHV08_RS13450 (IHV08_13450) holA 2583081..2584124 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  IHV08_RS13455 (IHV08_13455) - 2584164..2584313 (-) 150 WP_003229985.1 hypothetical protein -
  IHV08_RS13460 (IHV08_13460) yqzM 2584354..2584488 (+) 135 WP_003229983.1 YqzM family protein -
  IHV08_RS13465 (IHV08_13465) comEC 2584528..2586858 (-) 2331 WP_181217197.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  IHV08_RS13470 (IHV08_13470) comEB 2586862..2587431 (-) 570 WP_003229978.1 ComE operon protein 2 -
  IHV08_RS13475 (IHV08_13475) comEA 2587498..2588115 (-) 618 WP_033884458.1 competence protein ComEA Machinery gene
  IHV08_RS13480 (IHV08_13480) comER 2588199..2589020 (+) 822 WP_014480313.1 late competence protein ComER -
  IHV08_RS13485 (IHV08_13485) yqeM 2589086..2589829 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  IHV08_RS13490 (IHV08_13490) rsfS 2589826..2590182 (-) 357 WP_124072951.1 ribosome silencing factor -
  IHV08_RS13495 (IHV08_13495) yqeK 2590200..2590760 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  IHV08_RS13500 (IHV08_13500) nadD 2590750..2591319 (-) 570 WP_021480066.1 nicotinate-nucleotide adenylyltransferase -
  IHV08_RS13505 (IHV08_13505) yhbY 2591331..2591621 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  IHV08_RS13510 (IHV08_13510) aroE 2591615..2592457 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21736.47 Da        Isoelectric Point: 4.8354

>NTDB_id=490461 IHV08_RS13475 WP_033884458.1 2587498..2588115(-) (comEA) [Bacillus subtilis strain CV16]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESNETIVIDIKGAVRHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=490461 IHV08_RS13475 WP_033884458.1 2587498..2588115(-) (comEA) [Bacillus subtilis strain CV16]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAATGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(63-118)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

37.104

100

0.4
  comEA Staphylococcus aureus N315

35.909

100

0.385