Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KFU78_RS03795 Genome accession   NZ_CP074047
Coordinates   774394..775095 (+) Length   233 a.a.
NCBI ID   WP_222898839.1    Uniprot ID   -
Organism   Escherichia coli strain PD7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 769394..780095
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU78_RS03790 (KFU78_03740) sslE 769637..774196 (+) 4560 WP_001469318.1 lipoprotein metalloprotease SslE -
  KFU78_RS03795 (KFU78_03745) pilD 774394..775095 (+) 702 WP_222898839.1 A24 family peptidase Machinery gene
  KFU78_RS03805 (KFU78_03755) gspC 775837..776508 (+) 672 Protein_749 type II secretion system protein GspC -
  KFU78_RS03810 (KFU78_03760) gspD 776538..778598 (+) 2061 WP_222898906.1 type II secretion system secretin GspD -
  KFU78_RS03815 (KFU78_03765) pilB/pilB1 778598..780091 (+) 1494 WP_000249348.1 type II secretion system ATPase GspE Machinery gene

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 25902.82 Da        Isoelectric Point: 8.1383

>NTDB_id=490425 KFU78_RS03795 WP_222898839.1 774394..775095(+) (pilD) [Escherichia coli strain PD7]
MLFDVFQQYPAAMPVLATVGGLIIGSFLNVVIWRYPIMLRQQMAEFHGEMPSVQSKISLALPRSHCPHCQQTIRIRDNIP
LLSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSTWLIAASVIDLDHQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFIAFYSLRWIAGIVLRKEALGMGDVLLFAALGSWVGPLSLPNVVMTPTY

Nucleotide


Download         Length: 702 bp        

>NTDB_id=490425 KFU78_RS03795 WP_222898839.1 774394..775095(+) (pilD) [Escherichia coli strain PD7]
ATGCTTTTTGATGTTTTTCAGCAATACCCTGCGGCGATGCCCGTCCTGGCAACCGTCGGAGGATTGATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCATGGTGAAATGCCGAGTGTGC
AGTCAAAAATAAGCCTTGCGCTACCGCGTTCGCACTGCCCACATTGTCAGCAGACCATCCGGATACGTGACAATATTCCG
CTGCTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGTGATTGTCAGGCGAAAATCAGCAAGCGTTATCCGTTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCA
CCTGGCTGATTGCCGCGAGCGTCATTGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACG
GGACTGATTGCGGCATGGGCGCAGCAGAGCCCGCTCACGCTACAAGATGCAGTTACCGGCGTCCTGGTGGGGTTTATCGC
TTTTTACTCCCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTATTGTTCGCTG
CGTTAGGTAGTTGGGTGGGGCCGTTATCACTGCCCAATGTGGTAATGACTCCAACTTATTGA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

47.845

99.571

0.476
  pilD Vibrio campbellii strain DS40M4

43.534

99.571

0.433
  pilD Acinetobacter nosocomialis M2

40.086

99.571

0.399
  pilD Acinetobacter baumannii D1279779

39.655

99.571

0.395
  pilD Neisseria gonorrhoeae MS11

39.738

98.283

0.391
  pilD Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.838

95.279

0.361