Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF725_RS02450 Genome accession   NZ_CP062412
Coordinates   455237..455734 (+) Length   165 a.a.
NCBI ID   WP_020978197.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NAS_AN_181     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 450237..460734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF725_RS02410 (IF725_02405) - 450281..451267 (+) 987 WP_000161314.1 ROK family glucokinase -
  IF725_RS02415 (IF725_02410) - 451267..451596 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF725_RS02420 (IF725_02415) - 451593..452216 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF725_RS02425 (IF725_02420) comGA 452268..453242 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  IF725_RS02430 (IF725_02425) comGB 453214..454284 (+) 1071 WP_000776401.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF725_RS02435 (IF725_02430) comGC 454298..454609 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF725_RS02440 (IF725_02435) comGD 454587..455033 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF725_RS02445 (IF725_02440) comGE 455020..455319 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  IF725_RS02450 (IF725_02445) comGF 455237..455734 (+) 498 WP_020978197.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF725_RS02455 (IF725_02450) - 455831..455977 (+) 147 WP_001789879.1 hypothetical protein -
  IF725_RS02460 (IF725_02455) - 455967..456491 (+) 525 WP_001015121.1 shikimate kinase -
  IF725_RS02465 (IF725_02460) gcvT 456650..457741 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  IF725_RS02470 (IF725_02465) gcvPA 457761..459107 (+) 1347 WP_000019681.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF725_RS02475 (IF725_02470) gcvPB 459100..460572 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19158.95 Da        Isoelectric Point: 10.1964

>NTDB_id=489739 IF725_RS02450 WP_020978197.1 455237..455734(+) (comGF) [Staphylococcus aureus strain NAS_AN_181]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILQKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=489739 IF725_RS02450 WP_020978197.1 455237..455734(+) (comGF) [Staphylococcus aureus strain NAS_AN_181]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACAGAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

96.364

100

0.964