Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   IF725_RS02425 Genome accession   NZ_CP062412
Coordinates   452268..453242 (+) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain NAS_AN_181     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 447268..458242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF725_RS02390 (IF725_02385) rpmG 447728..447877 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  IF725_RS02395 (IF725_02390) - 448086..448625 (+) 540 WP_000164546.1 5-formyltetrahydrofolate cyclo-ligase -
  IF725_RS02400 (IF725_02395) - 448637..450100 (+) 1464 WP_001017871.1 rhomboid family intramembrane serine protease -
  IF725_RS02405 (IF725_02400) - 450081..450284 (+) 204 WP_000087561.1 YqgQ family protein -
  IF725_RS02410 (IF725_02405) - 450281..451267 (+) 987 WP_000161314.1 ROK family glucokinase -
  IF725_RS02415 (IF725_02410) - 451267..451596 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF725_RS02420 (IF725_02415) - 451593..452216 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  IF725_RS02425 (IF725_02420) comGA 452268..453242 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  IF725_RS02430 (IF725_02425) comGB 453214..454284 (+) 1071 WP_000776401.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF725_RS02435 (IF725_02430) comGC 454298..454609 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  IF725_RS02440 (IF725_02435) comGD 454587..455033 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF725_RS02445 (IF725_02440) comGE 455020..455319 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  IF725_RS02450 (IF725_02445) comGF 455237..455734 (+) 498 WP_020978197.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF725_RS02455 (IF725_02450) - 455831..455977 (+) 147 WP_001789879.1 hypothetical protein -
  IF725_RS02460 (IF725_02455) - 455967..456491 (+) 525 WP_001015121.1 shikimate kinase -
  IF725_RS02465 (IF725_02460) gcvT 456650..457741 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=489734 IF725_RS02425 WP_000697220.1 452268..453242(+) (comGA) [Staphylococcus aureus strain NAS_AN_181]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=489734 IF725_RS02425 WP_000697220.1 452268..453242(+) (comGA) [Staphylococcus aureus strain NAS_AN_181]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGGCCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGCGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1