Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   KFU72_RS03650 Genome accession   NZ_CP073994
Coordinates   752422..753081 (-) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli strain MB1     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 747422..758081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU72_RS03620 (KFU72_03595) ygiN 748323..748637 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  KFU72_RS03625 (KFU72_03600) mdaB 748668..749249 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  KFU72_RS03630 - 749499..749978 (+) 480 WP_000065332.1 Hcp family type VI secretion system effector -
  KFU72_RS03635 - 749981..750691 (+) 711 WP_000834030.1 hypothetical protein -
  KFU72_RS03640 (KFU72_03610) ygiZ 750698..751030 (+) 333 WP_000914690.1 DUF2645 family protein -
  KFU72_RS03645 (KFU72_03615) qseC 751076..752425 (-) 1350 WP_000673358.1 quorum sensing histidine kinase QseC -
  KFU72_RS03650 (KFU72_03620) ciaR 752422..753081 (-) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  KFU72_RS03655 (KFU72_03625) ygiW 753233..753625 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  KFU72_RS03660 (KFU72_03630) ygiV 753678..754160 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  KFU72_RS03665 (KFU72_03635) mqsR 754365..754661 (+) 297 WP_000415585.1 type II toxin-antitoxin system toxin MqsR -
  KFU72_RS03670 (KFU72_03640) mqsA 754663..755058 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  KFU72_RS03675 (KFU72_03645) ygiS 755191..756798 (+) 1608 WP_001375094.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=489467 KFU72_RS03650 WP_001221502.1 752422..753081(-) (ciaR) [Escherichia coli strain MB1]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=489467 KFU72_RS03650 WP_001221502.1 752422..753081(-) (ciaR) [Escherichia coli strain MB1]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365