Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   KFU73_RS17815 Genome accession   NZ_CP073974
Coordinates   3672063..3672722 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain MB22     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3667063..3677722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFU73_RS17790 (KFU73_17620) parE 3667154..3669046 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  KFU73_RS17795 (KFU73_17625) ygiN 3669094..3669408 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  KFU73_RS17800 (KFU73_17630) mdaB 3669439..3670020 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  KFU73_RS17805 (KFU73_17635) ygiZ 3670339..3670671 (+) 333 WP_112919162.1 DUF2645 family protein -
  KFU73_RS17810 (KFU73_17640) qseC 3670717..3672066 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  KFU73_RS17815 (KFU73_17645) ciaR 3672063..3672722 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  KFU73_RS17820 (KFU73_17650) ygiW 3672874..3673266 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  KFU73_RS17825 (KFU73_17655) ygiV 3673319..3673801 (+) 483 WP_000183504.1 GyrI-like domain-containing protein -
  KFU73_RS17830 (KFU73_17660) parC 3674347..3676605 (+) 2259 WP_001586259.1 DNA topoisomerase IV subunit A -
  KFU73_RS17835 (KFU73_17665) plsC 3676839..3677576 (+) 738 WP_000965722.1 1-acylglycerol-3-phosphate O-acyltransferase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=489119 KFU73_RS17815 WP_001221493.1 3672063..3672722(-) (ciaR) [Escherichia coli strain MB22]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=489119 KFU73_RS17815 WP_001221493.1 3672063..3672722(-) (ciaR) [Escherichia coli strain MB22]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365