Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF702_RS02300 Genome accession   NZ_CP062384
Coordinates   428348..428845 (+) Length   165 a.a.
NCBI ID   WP_001796472.1    Uniprot ID   A0A5F0HJ55
Organism   Staphylococcus aureus strain NAS_AN_050     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 423348..433845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF702_RS02260 (IF702_02255) - 423392..424378 (+) 987 WP_000161307.1 ROK family glucokinase -
  IF702_RS02265 (IF702_02260) - 424378..424707 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF702_RS02270 (IF702_02265) - 424704..425327 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  IF702_RS02275 (IF702_02270) comGA 425379..426353 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  IF702_RS02280 (IF702_02275) comGB 426325..427395 (+) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF702_RS02285 (IF702_02280) comGC 427409..427720 (+) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  IF702_RS02290 (IF702_02285) comGD 427698..428144 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF702_RS02295 (IF702_02290) comGE 428131..428430 (+) 300 WP_240024021.1 competence protein ComGE Machinery gene
  IF702_RS02300 (IF702_02295) comGF 428348..428845 (+) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF702_RS02305 (IF702_02300) - 428942..429088 (+) 147 WP_001789879.1 hypothetical protein -
  IF702_RS02310 (IF702_02305) - 429078..429602 (+) 525 WP_001015120.1 shikimate kinase -
  IF702_RS02315 (IF702_02310) gcvT 429761..430852 (+) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  IF702_RS02320 (IF702_02315) gcvPA 430872..432218 (+) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF702_RS02325 (IF702_02320) gcvPB 432211..433683 (+) 1473 WP_000202176.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19169.89 Da        Isoelectric Point: 10.1358

>NTDB_id=488903 IF702_RS02300 WP_001796472.1 428348..428845(+) (comGF) [Staphylococcus aureus strain NAS_AN_050]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=488903 IF702_RS02300 WP_001796472.1 428348..428845(+) (comGF) [Staphylococcus aureus strain NAS_AN_050]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

95.758

100

0.958