Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   IF702_RS02280 Genome accession   NZ_CP062384
Coordinates   426325..427395 (+) Length   356 a.a.
NCBI ID   WP_000776409.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NAS_AN_050     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 421325..432395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF702_RS02250 (IF702_02245) - 421748..423211 (+) 1464 WP_001017870.1 rhomboid family intramembrane serine protease -
  IF702_RS02255 (IF702_02250) - 423192..423395 (+) 204 WP_000087561.1 YqgQ family protein -
  IF702_RS02260 (IF702_02255) - 423392..424378 (+) 987 WP_000161307.1 ROK family glucokinase -
  IF702_RS02265 (IF702_02260) - 424378..424707 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF702_RS02270 (IF702_02265) - 424704..425327 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  IF702_RS02275 (IF702_02270) comGA 425379..426353 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  IF702_RS02280 (IF702_02275) comGB 426325..427395 (+) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF702_RS02285 (IF702_02280) comGC 427409..427720 (+) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  IF702_RS02290 (IF702_02285) comGD 427698..428144 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF702_RS02295 (IF702_02290) comGE 428131..428430 (+) 300 WP_240024021.1 competence protein ComGE Machinery gene
  IF702_RS02300 (IF702_02295) comGF 428348..428845 (+) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF702_RS02305 (IF702_02300) - 428942..429088 (+) 147 WP_001789879.1 hypothetical protein -
  IF702_RS02310 (IF702_02305) - 429078..429602 (+) 525 WP_001015120.1 shikimate kinase -
  IF702_RS02315 (IF702_02310) gcvT 429761..430852 (+) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  IF702_RS02320 (IF702_02315) gcvPA 430872..432218 (+) 1347 WP_000019698.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41536.00 Da        Isoelectric Point: 10.1113

>NTDB_id=488899 IF702_RS02280 WP_000776409.1 426325..427395(+) (comGB) [Staphylococcus aureus strain NAS_AN_050]
MKLQWINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGITILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNFTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVRQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=488899 IF702_RS02280 WP_000776409.1 426325..427395(+) (comGB) [Staphylococcus aureus strain NAS_AN_050]
GTGAAACTACAATGGATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGCATCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAACCAGATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATCATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTCTCTATTTTTA
TTGCTATGATTATAATATTAAACTTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACTAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAAATTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CGGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTACCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGAAAGCTAGAAGTAGAACTAAAGCTATATTCGCAAATATTAGTGAGGCAAATAGAGGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-146)

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

98.876

100

0.989
  comGB Staphylococcus aureus N315

98.876

100

0.989