Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   IF764_RS02300 Genome accession   NZ_CP062326
Coordinates   428296..428793 (+) Length   165 a.a.
NCBI ID   WP_001796472.1    Uniprot ID   A0A5F0HJ55
Organism   Staphylococcus aureus strain NAS_OP_043     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 423296..433793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IF764_RS02260 (IF764_02255) - 423340..424326 (+) 987 WP_000161307.1 ROK family glucokinase -
  IF764_RS02265 (IF764_02260) - 424326..424655 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  IF764_RS02270 (IF764_02265) - 424652..425275 (+) 624 WP_001223009.1 MBL fold metallo-hydrolase -
  IF764_RS02275 (IF764_02270) comGA 425327..426301 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  IF764_RS02280 (IF764_02275) comGB 426273..427343 (+) 1071 WP_000776409.1 competence type IV pilus assembly protein ComGB Machinery gene
  IF764_RS02285 (IF764_02280) comGC 427357..427668 (+) 312 WP_000472255.1 competence type IV pilus major pilin ComGC Machinery gene
  IF764_RS02290 (IF764_02285) comGD 427646..428092 (+) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  IF764_RS02295 (IF764_02290) comGE 428079..428378 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  IF764_RS02300 (IF764_02295) comGF 428296..428793 (+) 498 WP_001796472.1 competence type IV pilus minor pilin ComGF Machinery gene
  IF764_RS02305 (IF764_02300) - 428890..429036 (+) 147 WP_001789879.1 hypothetical protein -
  IF764_RS02310 (IF764_02305) - 429026..429550 (+) 525 WP_001015120.1 shikimate kinase -
  IF764_RS02315 (IF764_02310) gcvT 429709..430800 (+) 1092 WP_000093344.1 glycine cleavage system aminomethyltransferase GcvT -
  IF764_RS02320 (IF764_02315) gcvPA 430820..432166 (+) 1347 WP_240029576.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  IF764_RS02325 (IF764_02320) gcvPB 432159..433631 (+) 1473 WP_000202176.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19169.89 Da        Isoelectric Point: 10.1358

>NTDB_id=487783 IF764_RS02300 WP_001796472.1 428296..428793(+) (comGF) [Staphylococcus aureus strain NAS_OP_043]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=487783 IF764_RS02300 WP_001796472.1 428296..428793(+) (comGF) [Staphylococcus aureus strain NAS_OP_043]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5F0HJ55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

95.758

100

0.958