Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   KEW59_RS14500 Genome accession   NZ_CP073621
Coordinates   3060799..3061458 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain CE2050     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3055799..3066458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KEW59_RS14470 (KEW59_14355) - 3055914..3056861 (+) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -
  KEW59_RS14475 (KEW59_14360) - 3056858..3057745 (+) 888 WP_001596209.1 MurR/RpiR family transcriptional regulator -
  KEW59_RS14480 (KEW59_14365) ygiN 3057790..3058104 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  KEW59_RS14485 (KEW59_14370) mdaB 3058135..3058716 (-) 582 WP_000072420.1 NADPH:quinone oxidoreductase MdaB -
  KEW59_RS14490 (KEW59_14375) ygiZ 3059075..3059404 (+) 330 WP_001551659.1 DUF2645 family protein -
  KEW59_RS14495 (KEW59_14380) qseC 3059453..3060802 (-) 1350 WP_000673373.1 quorum sensing histidine kinase QseC -
  KEW59_RS14500 (KEW59_14385) ciaR 3060799..3061458 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  KEW59_RS14505 (KEW59_14390) ygiW 3061610..3062002 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  KEW59_RS14510 (KEW59_14395) ygiV 3062055..3062537 (+) 483 WP_000183492.1 GyrI-like domain-containing protein -
  KEW59_RS14515 (KEW59_14400) ygiS 3062646..3064253 (+) 1608 WP_001596207.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=487443 KEW59_RS14500 WP_001221493.1 3060799..3061458(-) (ciaR) [Escherichia coli strain CE2050]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=487443 KEW59_RS14500 WP_001221493.1 3060799..3061458(-) (ciaR) [Escherichia coli strain CE2050]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTAGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365