Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   KD154_RS11945 Genome accession   NZ_CP073589
Coordinates   2450795..2451454 (+) Length   219 a.a.
NCBI ID   WP_001221499.1    Uniprot ID   -
Organism   Escherichia coli strain MFDS1006657     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2445795..2456454
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KD154_RS11925 plsC 2445942..2446679 (-) 738 WP_000965712.1 1-acylglycerol-3-phosphate O-acyltransferase -
  KD154_RS11930 parC 2446912..2449170 (-) 2259 WP_001281881.1 DNA topoisomerase IV subunit A -
  KD154_RS11935 ygiV 2449716..2450198 (-) 483 WP_000183481.1 GyrI-like domain-containing protein -
  KD154_RS11940 ygiW 2450251..2450643 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  KD154_RS11945 ciaR 2450795..2451454 (+) 660 WP_001221499.1 quorum sensing response regulator transcription factor QseB Regulator
  KD154_RS11950 qseC 2451451..2452800 (+) 1350 WP_000673354.1 quorum sensing histidine kinase QseC -
  KD154_RS11955 ygiZ 2452846..2453178 (-) 333 WP_000912120.1 DUF2645 family protein -
  KD154_RS11960 mdaB 2453497..2454078 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  KD154_RS11965 ygiN 2454109..2454423 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  KD154_RS11970 parE 2454471..2456363 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24731.64 Da        Isoelectric Point: 6.6540

>NTDB_id=487348 KD154_RS11945 WP_001221499.1 2450795..2451454(+) (ciaR) [Escherichia coli strain MFDS1006657]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGDPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=487348 KD154_RS11945 WP_001221499.1 2450795..2451454(+) (ciaR) [Escherichia coli strain MFDS1006657]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGACCCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365