Detailed information NTG0486979

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KCG55_RS04075
• Genome accession: NZ_CP073117
• Coordinates: 815920..817149 (+)
• Length: 409 a.a.
• NCBI ID: WP_254323444.1
• Uniprot ID: -
• Organism: Neisseria subflava strain HP0015
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 810920..822149

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KCG55_RS04045 (KCG55_04050)	dksA	811466..811882 (-)	417	WP_049350731.1	RNA polymerase-binding protein DksA	-
KCG55_RS04050 (KCG55_04055)	-	812116..812673 (-)	558	WP_254323440.1	YggT family protein	-
KCG55_RS04055 (KCG55_04060)	proC	812676..813473 (-)	798	WP_254323441.1	pyrroline-5-carboxylate reductase	-
KCG55_RS04060 (KCG55_04065)	-	813564..813959 (-)	396	WP_254323442.1	RNA-binding protein	-
KCG55_RS04065 (KCG55_04070)	-	813956..814669 (-)	714	WP_254323443.1	YggS family pyridoxal phosphate-dependent enzyme	-
KCG55_RS04070 (KCG55_04075)	pilT	814787..815833 (+)	1047	WP_004519239.1	type IV pilus twitching motility protein PilT	Machinery gene
KCG55_RS04075 (KCG55_04080)	pilU	815920..817149 (+)	1230	WP_254323444.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KCG55_RS04080 (KCG55_04085)	yccS	817316..819451 (+)	2136	WP_254323445.1	YccS family putative transporter	-
KCG55_RS04085 (KCG55_04090)	-	819615..820793 (-)	1179	WP_254323446.1	phosphoglycerate kinase	-

Sequence

Protein

• Length: 409 a.a.
• Molecular weight: 45847.31 Da
• Isoelectric Point: 6.1852

>NTDB_id=486979 KCG55_RS04075 WP_254323444.1 815920..817149(+) (pilU) [Neisseria subflava strain HP0015]
MSDLAPLHDLLSEMVQAYSQKNQAPHIPTPAEIGTHLHPLLDRMCEEAEKRNASDIFISAGFPPAIKVGGTLTPMPHKAL
SGADTAAIAESTMNPDQLEAFNRYWEINYSVQSRSNTRYRVNAYHEQGRVGMVLRRVSQDIPEMKTLGLPEKLQELALAP
RGLLILAGPTGSGKSTTMASMLNYRNQKLPGHIVTIEDPIEFIYKPCRSIFTQREIGIDTDNWKTAVQSAMRQAPDVVCI
GEVRSEESIEYALQLAQTGHLCVFTIHASTASQTIERIINFYPEDRRQQVLMDLALNLVGIIGQRLTTKKNRQRTAVTDL
LLNTPAMQDLIFKGELLEIRDLMARSGSNGMQTFDQNLFNLYAQGQIELSEALRQAVSANDLRLRIQMHDEGNNTERLYD
RISDLNLMT

Nucleotide

• Length: 1230 bp

>NTDB_id=486979 KCG55_RS04075 WP_254323444.1 815920..817149(+) (pilU) [Neisseria subflava strain HP0015]
ATGAGCGACCTCGCCCCTCTGCACGACCTGTTAAGCGAAATGGTGCAAGCCTACTCACAAAAAAACCAAGCACCGCACAT
CCCCACCCCGGCCGAAATCGGCACACATCTTCACCCCTTGCTCGACCGCATGTGTGAAGAGGCTGAAAAACGCAACGCCT
CCGACATCTTCATCAGCGCAGGCTTTCCCCCTGCCATCAAAGTCGGCGGCACCCTCACGCCCATGCCCCACAAAGCGCTG
TCCGGCGCGGACACCGCCGCCATCGCCGAATCTACCATGAACCCCGACCAGCTCGAAGCCTTCAACCGCTACTGGGAAAT
CAACTATTCCGTCCAATCGCGCAGCAACACGCGCTACCGCGTCAACGCCTACCACGAGCAAGGCCGCGTCGGCATGGTAT
TGCGCCGCGTCAGCCAAGACATTCCCGAAATGAAGACCCTCGGTTTGCCCGAAAAACTCCAAGAGCTTGCACTCGCGCCG
CGCGGCCTGCTGATCCTCGCCGGCCCAACCGGTTCGGGTAAATCCACCACCATGGCCTCCATGCTCAACTACCGCAACCA
AAAGCTGCCCGGCCATATCGTCACCATCGAAGACCCCATCGAGTTTATCTACAAACCTTGCCGTAGCATCTTCACCCAGC
GCGAAATCGGCATCGATACCGACAACTGGAAAACCGCCGTTCAAAGCGCCATGCGTCAAGCACCCGATGTCGTCTGTATC
GGCGAAGTCCGCAGCGAAGAAAGCATAGAATACGCCCTCCAACTGGCTCAAACCGGCCATCTGTGCGTTTTCACCATCCA
TGCCAGCACAGCTTCCCAAACCATCGAGCGCATTATCAACTTCTATCCGGAAGACCGCCGCCAACAGGTTTTGATGGATT
TGGCGTTAAACCTTGTCGGCATCATCGGCCAACGCCTCACCACCAAGAAAAACCGCCAACGCACGGCCGTTACCGACCTC
CTGCTCAATACTCCGGCCATGCAGGACTTGATTTTCAAAGGCGAATTGCTGGAAATCCGCGACCTGATGGCACGCTCCGG
CAGTAACGGCATGCAGACGTTTGACCAAAACCTCTTCAACCTCTACGCACAAGGCCAAATCGAACTCAGCGAAGCCCTGC
GCCAAGCGGTATCTGCCAACGATTTGCGCCTGCGCATTCAAATGCACGACGAGGGCAACAATACCGAACGCCTCTACGAC
CGCATCAGCGACCTCAACCTGATGACCTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (132-309)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	47.324	86.797	0.411
pilU	Pseudomonas stutzeri DSM 10701	47.813	83.863	0.401
pilU	Vibrio cholerae strain A1552	47.181	82.396	0.389