Detailed information NTG0486942

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KCG56_RS09520
• Genome accession: NZ_CP073116
• Coordinates: 1948003..1949232 (+)
• Length: 409 a.a.
• NCBI ID: WP_254321254.1
• Uniprot ID: -
• Organism: Neisseria subflava strain TT0073
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1943003..1954232

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KCG56_RS09495 (KCG56_09545)	dksA	1943973..1944389 (-)	417	WP_254321250.1	RNA polymerase-binding protein DksA	-
KCG56_RS09500 (KCG56_09550)	-	1944623..1945180 (-)	558	WP_254321251.1	YggT family protein	-
KCG56_RS09505 (KCG56_09555)	proC	1945183..1945980 (-)	798	WP_254321252.1	pyrroline-5-carboxylate reductase	-
KCG56_RS09510 (KCG56_09560)	-	1946057..1946752 (-)	696	WP_254321253.1	YggS family pyridoxal phosphate-dependent enzyme	-
KCG56_RS09515 (KCG56_09565)	pilT	1946870..1947916 (+)	1047	WP_004519239.1	type IV pilus twitching motility protein PilT	Machinery gene
KCG56_RS09520 (KCG56_09570)	pilU	1948003..1949232 (+)	1230	WP_254321254.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KCG56_RS09525 (KCG56_09575)	yccS	1949398..1951533 (+)	2136	WP_254321255.1	YccS family putative transporter	-
KCG56_RS09530 (KCG56_09580)	-	1951699..1952877 (-)	1179	WP_254321256.1	phosphoglycerate kinase	-

Sequence

Protein

• Length: 409 a.a.
• Molecular weight: 45843.34 Da
• Isoelectric Point: 6.0120

>NTDB_id=486942 KCG56_RS09520 WP_254321254.1 1948003..1949232(+) (pilU) [Neisseria subflava strain TT0073]
MSDLAPLHDLLSEMVQAYSQKNQAPHIPTPAEIGTHLHPLLDRMCEEAEKRNASDIFISAGFPPAIKVGGTLTPMPHKAL
TGADTAAIAESTMNPDQLEAFNRDWEINYSVQSRSNTRYRVNAYHEQGRVGMVLRRVSQDIPEMKTLGLPEKLQELALAP
RGLLILAGPTGSGKSTTMASMLNYRNQKLPGHIVTIEDPIEFIYKPCRSIFTQREIGIDTDNWKIAVQSAMRQAPDVVCI
GEVRSEESMEYALQLAQTGHLCVFTIHASTASQTIERIINFYPEDRRQQVLMDLALNLVGIIGQRLTTKKNRQRTAVTDL
LLNTPAMQDLIFKGELLEIRDLMARSGSNGMQTFDQNLFNLYAQGQIELSEALRQAVSANDLRLRIQMHDEGNNTERLYD
RISDLNLMT

Nucleotide

• Length: 1230 bp

>NTDB_id=486942 KCG56_RS09520 WP_254321254.1 1948003..1949232(+) (pilU) [Neisseria subflava strain TT0073]
ATGAGCGACCTCGCCCCTTTGCACGACCTGTTAAGCGAAATGGTGCAAGCCTACTCACAAAAAAACCAAGCACCGCACAT
CCCCACTCCGGCCGAAATCGGCACGCATCTTCATCCCCTGCTCGACCGTATGTGTGAAGAGGCCGAAAAACGCAACGCAT
CCGACATCTTCATCAGCGCAGGCTTTCCCCCTGCCATCAAAGTCGGCGGCACCCTCACGCCCATGCCCCACAAAGCTTTG
ACCGGCGCGGACACCGCTGCCATCGCCGAATCCACCATGAACCCCGACCAGCTCGAAGCCTTTAACCGCGACTGGGAAAT
CAACTATTCCGTCCAATCGCGCAGCAACACGCGCTACCGCGTCAACGCCTACCACGAGCAAGGCCGCGTCGGCATGGTAT
TGCGCCGCGTCAGCCAAGACATTCCCGAAATGAAGACACTCGGCTTACCCGAAAAGCTCCAAGAACTTGCTCTTGCACCG
CGCGGTCTGCTGATCCTCGCCGGCCCGACCGGTTCGGGTAAATCCACCACCATGGCCTCCATGCTCAACTACCGCAACCA
AAAGCTGCCCGGCCATATCGTTACCATCGAAGACCCCATCGAGTTTATCTACAAACCTTGCCGCAGCATCTTCACCCAGC
GAGAAATCGGCATCGATACCGACAACTGGAAAATCGCCGTTCAAAGCGCCATGCGTCAAGCACCCGACGTCGTCTGTATC
GGCGAAGTCCGCAGCGAAGAAAGCATGGAATACGCCCTACAACTGGCTCAAACCGGCCATCTGTGCGTGTTTACCATCCA
TGCCAGCACAGCCTCCCAAACCATCGAGCGCATCATCAACTTCTATCCGGAAGACCGCCGCCAACAGGTTTTAATGGATT
TAGCGTTAAACCTCGTCGGCATCATCGGCCAACGCCTCACCACCAAGAAAAACCGCCAACGTACGGCCGTTACCGACCTC
CTGCTCAACACTCCGGCCATGCAGGACTTGATTTTCAAAGGCGAATTGCTGGAAATCCGCGACCTGATGGCACGCTCCGG
CAGCAACGGCATGCAGACATTCGACCAAAACCTCTTCAACCTCTACGCACAAGGCCAAATCGAACTCAGCGAAGCCCTGC
GCCAAGCGGTTTCCGCCAACGATTTGCGCCTGCGCATCCAAATGCACGACGAGGGCAACAATACCGAGCGTCTCTACGAC
CGCATCAGCGACCTCAACCTGATGACCTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (130-309)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	47.887	86.797	0.416
pilU	Pseudomonas stutzeri DSM 10701	47.813	83.863	0.401
pilU	Vibrio cholerae strain A1552	47.181	82.396	0.389