Detailed information NTG0486165

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IG194_RS15620
• Genome accession: NZ_CP062122
• Coordinates: 3443149..3444213 (+)
• Length: 354 a.a.
• NCBI ID: WP_009613650.1
• Uniprot ID: A0A1A5D9E0
• Organism: Pseudomonas sp. ADPe
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3438149..3449213

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IG194_RS15600 (IG194_15605)	-	3438237..3438659 (+)	423	WP_058488149.1	hypothetical protein	-
IG194_RS15605 (IG194_15610)	-	3438702..3439472 (+)	771	WP_058488150.1	class I SAM-dependent methyltransferase	-
IG194_RS15610 (IG194_15615)	-	3439534..3440025 (+)	492	WP_058488151.1	M48 family metallopeptidase	-
IG194_RS15615 (IG194_15620)	-	3440531..3442963 (-)	2433	WP_043268466.1	penicillin-binding protein 1A	-
IG194_RS15620 (IG194_15625)	pilM	3443149..3444213 (+)	1065	WP_009613650.1	pilus assembly protein PilM	Machinery gene
IG194_RS15625 (IG194_15630)	pilN	3444213..3444812 (+)	600	WP_009613648.1	type 4a pilus biogenesis protein PilN	-
IG194_RS15630 (IG194_15635)	pilO	3444809..3445465 (+)	657	WP_009613645.1	type 4a pilus biogenesis protein PilO	-
IG194_RS15635 (IG194_15640)	pilP	3445462..3445986 (+)	525	WP_009613643.1	type 4a pilus biogenesis lipoprotein PilP	-
IG194_RS15640 (IG194_15645)	pilQ	3446043..3448136 (+)	2094	WP_058488152.1	type IV pilus secretin PilQ	Machinery gene
IG194_RS15645 (IG194_15650)	aroK	3448145..3448663 (+)	519	WP_024128057.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37773.09 Da
• Isoelectric Point: 4.4526

>NTDB_id=486165 IG194_RS15620 WP_009613650.1 3443149..3444213(+) (pilM) [Pseudomonas sp. ADPe]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNSVVEKNIAELEGVGQALSRVVAKARTGVKTAAV
AVAGSAVITKTIEMDGGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNAERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAFGLLGDQLGGNADELTVAVVDIGATMTTLSVLHHGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMVLSNKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=486165 IG194_RS15620 WP_009613650.1 3443149..3444213(+) (pilM) [Pseudomonas sp. ADPe]
GTGCTAGGGCTCTTCACTAAGAAAGCGAACACACTGCTGGGGATCGACATCAGTTCGACTTCGGTCAAACTCCTCGAGTT
GAGCCGTTCGGGCGGGCGCTACAAGGTAGAGGCCTACGCCGTAGAGCCGCTCCCGCCGAACTCGGTGGTCGAGAAAAACA
TCGCCGAACTGGAGGGGGTCGGCCAGGCGCTGTCGCGGGTGGTCGCCAAGGCGCGCACCGGCGTGAAGACGGCTGCCGTG
GCGGTCGCCGGCTCGGCGGTCATCACCAAGACCATCGAGATGGACGGCGGGCTTTCCGAAGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCCGACCAGTACATTCCCTATCCCCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGCGTATCCGCGC
GCAACGCCGAGCGCGTCGACGTGCTGCTGGCGGCCTGCCGCAAGGAGAACGTCGAGGTGCGCGAGGCCGCGCTGGCCCTG
GCCGGGCTCACTGCCAAGGTGGTGGACGTCGAGGCCTACGCGCTGGAGCGCGCCTTCGGCCTGCTCGGCGACCAGTTGGG
CGGCAACGCCGACGAGCTGACCGTCGCGGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTCCACCACGGCC
GCACCATCTATACCCGCGAGCAGCTGTTCGGCGGGCGCCAGCTCACCGAGGAGATCCAGCGCCGCTATGGCCTGTCGGTG
GAGGAAGCCGGCCTTGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTGTTGCAACCGTTCAAGGATGC
GGTGGTGCAGCAGGTCTCGCGTTCGCTGCAGTTCTTCTTCGCCGCTGGCCAGTTCAACGACGTCGACTACATCCTGCTCG
CTGGCGGCACGGCTTCCATCCCCGACCTGGACCGGCTCATCCAGCAGAAGATCGGCACCCCGACGCTGGTGGCCAACCCC
TTCGCCGACATGGTGCTGAGCAACAAGGTGAATGCCGGGGCGCTGGCCAGTGATGCGCCGGCGCTGATGATTGCCTGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1A5D9E0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.345	100	0.573
comM	Acinetobacter nosocomialis M2	57.345	100	0.573
comM	Acinetobacter baylyi ADP1	53.39	100	0.534
pilM	Legionella pneumophila strain ERS1305867	46.045	100	0.46