Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   IG194_RS15640 Genome accession   NZ_CP062122
Coordinates   3446043..3448136 (+) Length   697 a.a.
NCBI ID   WP_058488152.1    Uniprot ID   A0A1A5DA22
Organism   Pseudomonas sp. ADPe     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3441043..3453136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IG194_RS15620 (IG194_15625) pilM 3443149..3444213 (+) 1065 WP_009613650.1 pilus assembly protein PilM Machinery gene
  IG194_RS15625 (IG194_15630) pilN 3444213..3444812 (+) 600 WP_009613648.1 type 4a pilus biogenesis protein PilN -
  IG194_RS15630 (IG194_15635) pilO 3444809..3445465 (+) 657 WP_009613645.1 type 4a pilus biogenesis protein PilO -
  IG194_RS15635 (IG194_15640) pilP 3445462..3445986 (+) 525 WP_009613643.1 type 4a pilus biogenesis lipoprotein PilP -
  IG194_RS15640 (IG194_15645) pilQ 3446043..3448136 (+) 2094 WP_058488152.1 type IV pilus secretin PilQ Machinery gene
  IG194_RS15645 (IG194_15650) aroK 3448145..3448663 (+) 519 WP_024128057.1 shikimate kinase AroK -
  IG194_RS15650 (IG194_15655) aroB 3448715..3449821 (+) 1107 WP_043313186.1 3-dehydroquinate synthase -
  IG194_RS15655 (IG194_15660) - 3449828..3451504 (+) 1677 WP_043268470.1 AAA family ATPase -

Sequence

Protein


Download         Length: 697 a.a.        Molecular weight: 74924.05 Da        Isoelectric Point: 5.2731

>NTDB_id=486166 IG194_RS15640 WP_058488152.1 3446043..3448136(+) (pilQ) [Pseudomonas sp. ADPe]
MNRTMTRLGFALLAGLFAPSLLAANLQRVDVASLPGDRVELKLGFDEPVAAPRGYTIEQPARIALDLPGVQNKLGSKNRE
LGVGNARSLTVVEAKDRTRLIINLTTLVPYSTRAQGNDLIILVGQGGGAPAAAASSAPSAAPAYAAAPRPAQPKPYVPVG
KAIRNIDFQRGEQGEGNVVIDLSDPSVSPDIQEQGGKIRLDFPRTQLPDSLRVRLDVKDFATPVQFVNASAAGDKASIVI
EPSGLYDYLVFQTDNRMTVSIKPMTANEAEKRKKDSFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKASDIAKLFQSVT
GTSSGPGAQPAAGTDNRGSITVDDRTNAIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEASVDYTKELGVQWGGSL
NNRGNFNITGGTGGTSSANTFVDLGVSTASPTSGIGIGFVTDNTTLDLQLNAMEKTGNGEVVSQPKVVTSDKETAKILKG
SEIPYQEASSSGATSVSFKEAALSLEVTPQITPDNRIIMQVKVNKDEPDYQNALLGVPPIKKNEVNAKVLVNDGETIVIG
GVFSNTQTKTTSKVPFLGDVPVVGRLFKNDYVTDTKNELLVFLTPRIMNNQAIAIDR

Nucleotide


Download         Length: 2094 bp        

>NTDB_id=486166 IG194_RS15640 WP_058488152.1 3446043..3448136(+) (pilQ) [Pseudomonas sp. ADPe]
ATGAACAGAACTATGACCCGCCTGGGCTTTGCCCTGTTGGCCGGCCTTTTCGCGCCGAGCCTGCTCGCCGCCAACCTGCA
GCGCGTCGACGTGGCCTCGCTGCCGGGGGATCGCGTGGAGCTCAAGCTGGGCTTCGACGAGCCGGTGGCCGCGCCGCGTG
GCTACACCATCGAGCAGCCGGCGCGCATCGCCCTGGACCTGCCGGGCGTGCAGAACAAGCTGGGCAGCAAGAACCGCGAG
CTGGGCGTGGGCAACGCGCGCAGCCTGACCGTGGTCGAGGCCAAGGACCGCACCCGCCTGATCATCAACCTGACCACCCT
GGTGCCGTATTCCACCCGCGCCCAGGGCAACGACCTGATCATCCTGGTTGGCCAGGGGGGCGGCGCGCCCGCCGCGGCGG
CCAGCTCCGCACCGAGCGCGGCCCCGGCCTATGCCGCGGCGCCTCGCCCGGCGCAGCCCAAGCCCTACGTGCCGGTGGGC
AAGGCGATCCGCAACATCGACTTCCAGCGCGGCGAGCAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGAGCGT
CAGCCCGGACATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCCCGCGCACCCAGCTGCCCGACAGCCTGCGCGTGC
GCCTGGACGTGAAGGACTTCGCCACCCCGGTGCAGTTCGTCAACGCCTCGGCGGCCGGCGACAAGGCCAGCATCGTCATC
GAGCCCAGCGGCCTGTACGACTACCTGGTGTTCCAGACCGACAACCGCATGACCGTCAGCATCAAGCCGATGACCGCCAA
CGAGGCCGAGAAGCGCAAGAAGGACAGCTTCGCCTACACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GCTCGGTGCTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGACACCGTGCAGGGCAACATCACCCTG
CGCCTGCAGAACGTGCCCTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGGCAA
CGTCCTGCTGGTGGCGCCGGCCGACGAGATCGCCGCGCGCGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAAC
TGGCGCCCCTGCGCCGCGAGCTGATCCAGGTGAACTACGCCAAGGCCTCCGACATCGCCAAGCTGTTCCAGTCCGTGACC
GGCACCAGTTCCGGCCCGGGCGCCCAGCCTGCCGCCGGCACCGACAACCGTGGCTCCATCACCGTCGACGACCGCACCAA
CGCCATCATCGCCTACCAGACCCAGGACCGCCTGGACGAGCTGCGCCGCATCGTCTCGCAGCTGGACATCCCGGTACGCC
AGGTGATGATCGAGGCGCGCATCGTCGAGGCCAGCGTCGACTACACCAAGGAGCTGGGGGTGCAGTGGGGTGGTTCGCTG
AATAATCGCGGCAACTTCAACATCACCGGTGGTACTGGTGGTACCAGCAGTGCGAATACCTTCGTCGATCTGGGCGTCAG
CACCGCGTCCCCGACCTCCGGTATCGGCATCGGCTTCGTCACCGACAACACCACCCTGGACCTGCAGCTCAACGCCATGG
AGAAGACCGGCAACGGCGAAGTGGTGTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGC
TCGGAGATTCCCTACCAGGAAGCCAGCTCCAGCGGCGCCACCTCGGTGTCCTTCAAGGAGGCCGCGCTGTCCCTGGAGGT
CACTCCGCAGATCACCCCGGACAACCGCATCATCATGCAGGTCAAGGTGAACAAGGACGAGCCCGACTACCAGAACGCGC
TGCTCGGCGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCAAGGTACTGGTCAACGACGGTGAAACCATCGTCATCGGC
GGGGTGTTCTCCAACACCCAGACCAAGACCACCTCCAAGGTCCCGTTCCTCGGCGACGTACCGGTCGTCGGCCGCCTGTT
CAAGAACGACTATGTGACGGACACCAAGAACGAGCTTCTGGTTTTCCTCACTCCCCGGATCATGAATAATCAGGCCATTG
CCATCGATCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-121)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II and III secretion system protein (Secretin)

(532-688)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1A5DA22

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.178

100

0.801
  pilQ Acinetobacter baumannii strain A118

40.673

100

0.416
  pilQ Acinetobacter baumannii D1279779

40.673

100

0.416
  comQ Acinetobacter baylyi ADP1

41.583

99.713

0.415
  pilQ Legionella pneumophila strain Lp02

39.385

97.991

0.386
  pilQ Legionella pneumophila strain ERS1305867

39.239

97.991

0.385