Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   KBW97_RS03460 Genome accession   NZ_CP072986
Coordinates   718512..719171 (-) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain T159A     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 713512..724171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBW97_RS03435 parE 713603..715495 (+) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -
  KBW97_RS03440 ygiN 715543..715857 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  KBW97_RS03445 mdaB 715888..716469 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  KBW97_RS03450 ygiZ 716788..717120 (+) 333 WP_000917684.1 DUF2645 family protein -
  KBW97_RS03455 qseC 717166..718515 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  KBW97_RS03460 ciaR 718512..719171 (-) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  KBW97_RS03465 ygiW 719323..719715 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  KBW97_RS03470 ygiV 719768..720250 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  KBW97_RS03475 mqsR 720455..720751 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  KBW97_RS03480 mqsA 720753..721148 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  KBW97_RS03485 ygiS 721281..722888 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=486083 KBW97_RS03460 WP_001221495.1 718512..719171(-) (ciaR) [Escherichia coli strain T159A]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=486083 KBW97_RS03460 WP_001221495.1 718512..719171(-) (ciaR) [Escherichia coli strain T159A]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361