Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   KBP48_RS10210 Genome accession   NZ_CP072932
Coordinates   2276217..2277080 (-) Length   287 a.a.
NCBI ID   WP_004084594.1    Uniprot ID   B2I9Q6
Organism   Xylella fastidiosa subsp. multiplex strain BB08-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2271217..2282080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBP48_RS10190 (KBP48_10190) rimK 2271709..2272614 (+) 906 WP_027700148.1 30S ribosomal protein S6--L-glutamate ligase -
  KBP48_RS10195 (KBP48_10195) - 2273308..2273985 (+) 678 WP_010894955.1 response regulator transcription factor -
  KBP48_RS10200 (KBP48_10200) - 2273978..2275315 (+) 1338 WP_027700147.1 HAMP domain-containing sensor histidine kinase -
  KBP48_RS10205 (KBP48_10205) coaE 2275582..2276205 (-) 624 WP_027700146.1 dephospho-CoA kinase -
  KBP48_RS10210 (KBP48_10210) pilD 2276217..2277080 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  KBP48_RS10215 (KBP48_10215) pilC 2277087..2278289 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  KBP48_RS10220 (KBP48_10220) pilA 2278730..2279176 (+) 447 WP_071869805.1 pilin Machinery gene
  KBP48_RS10225 (KBP48_10225) - 2279421..2279648 (-) 228 WP_012338102.1 hypothetical protein -
  KBP48_RS10230 (KBP48_10230) pilA/pilAI 2280481..2280924 (+) 444 WP_140189469.1 pilin Machinery gene

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31704.79 Da        Isoelectric Point: 8.6709

>NTDB_id=485837 KBP48_RS10210 WP_004084594.1 2276217..2277080(-) (pilD) [Xylella fastidiosa subsp. multiplex strain BB08-1]
MAFLDQHPGLGYPLASGLGLLLGSFLNVVILRLPRRLEWQWKRDAREILEMPDIYDPPPPGIVVEPSHDPVTGDRLKWWE
NIPVLSWLLLRGRSRYSGKSISLQYPLVELLTCLLVLASVWRFGFGWHGFGAIVFSCFLVALAGIDLRTRLLPDQLTLML
MWLGLIGSADNLFMAAKPALLGAAVGYLSLWIVAALFKLIFNKEGMGHGDFKMLGALGAWCGLKGILPIILISSLAGVVL
GSVWLVVRGHNRTTMIPFGPYLAVGGWIVFFFGDQIIHAYLELLSIG

Nucleotide


Download         Length: 864 bp        

>NTDB_id=485837 KBP48_RS10210 WP_004084594.1 2276217..2277080(-) (pilD) [Xylella fastidiosa subsp. multiplex strain BB08-1]
ATGGCATTTCTTGATCAGCACCCCGGACTCGGCTATCCCCTTGCGTCCGGTTTGGGGTTGTTGCTTGGTAGTTTTCTCAA
TGTGGTGATCCTGCGTTTGCCGCGCCGTTTGGAATGGCAATGGAAGCGCGATGCGCGCGAAATCCTGGAGATGCCGGACA
TTTATGATCCGCCACCACCGGGAATTGTGGTCGAGCCTTCCCACGATCCAGTGACTGGTGATCGGTTGAAGTGGTGGGAA
AACATTCCGGTGCTTAGTTGGTTGTTGCTACGTGGACGCTCGCGTTACAGCGGCAAGTCAATCTCGTTGCAGTATCCGTT
GGTCGAATTGCTGACTTGCCTGCTGGTGCTGGCCAGTGTTTGGCGTTTTGGATTCGGTTGGCATGGCTTTGGGGCAATTG
TGTTCAGTTGTTTCCTGGTTGCGTTGGCTGGGATTGATTTGCGTACTCGGCTCTTACCTGACCAGTTAACTTTAATGCTT
ATGTGGCTGGGTTTGATTGGATCTGCGGATAACTTGTTTATGGCAGCCAAACCTGCGTTATTGGGGGCTGCAGTTGGTTA
TTTATCTCTGTGGATTGTGGCGGCGTTGTTCAAACTGATCTTTAACAAGGAAGGGATGGGCCATGGTGACTTCAAAATGT
TGGGCGCGCTTGGGGCATGGTGTGGGTTGAAGGGTATTCTCCCGATCATTTTGATTTCATCACTGGCTGGAGTGGTATTG
GGCTCCGTTTGGCTTGTTGTGCGTGGTCATAACCGTACGACGATGATTCCGTTTGGACCTTATCTGGCAGTTGGTGGATG
GATTGTGTTCTTTTTCGGCGATCAGATCATCCATGCTTATCTGGAGCTATTAAGTATCGGGTGA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(133-241)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(18-123)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B2I9Q6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

41.754

99.303

0.415
  pilD Vibrio cholerae strain A1552

42.049

98.606

0.415
  pilD Acinetobacter nosocomialis M2

41.343

98.606

0.408
  pilD Acinetobacter baumannii D1279779

41.343

98.606

0.408
  pilD Neisseria gonorrhoeae MS11

43.284

93.38

0.404