NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	IEN91_RS13395	Genome accession	NZ_CP061938
Coordinates	2729956..2730570 (-)	Length	204 a.a.
NCBI ID	WP_015387983.1	Uniprot ID	A0A6A8LHQ3
Organism	Bacillus velezensis strain FIO1408
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2724956..2735570

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IEN91_RS13365 (IEN91_13365)	rpsT	2725256..2725522 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
IEN91_RS13370 (IEN91_13370)	holA	2725539..2726580 (-)	1042	Protein_2599	DNA polymerase III subunit delta	-
IEN91_RS13375 (IEN91_13375)	-	2726620..2726774 (-)	155	Protein_2600	hypothetical protein	-
IEN91_RS13380 (IEN91_13380)	-	2726815..2726949 (+)	135	WP_003152870.1	YqzM family protein	-
IEN91_RS13385 (IEN91_13385)	comEC	2726968..2729319 (-)	2352	WP_069448879.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
IEN91_RS13390 (IEN91_13390)	-	2729320..2729889 (-)	570	WP_003152868.1	ComE operon protein 2	-
IEN91_RS13395 (IEN91_13395)	comEA	2729956..2730570 (-)	615	WP_015387983.1	helix-hairpin-helix domain-containing protein	Machinery gene
IEN91_RS13400 (IEN91_13400)	comER	2730629..2731450 (+)	822	WP_014305449.1	late competence protein ComER	-
IEN91_RS13405 (IEN91_13405)	-	2731519..2732256 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
IEN91_RS13410 (IEN91_13410)	rsfS	2732253..2732609 (-)	357	WP_003152864.1	ribosome silencing factor	-
IEN91_RS13415 (IEN91_13415)	yqeK	2732606..2733187 (-)	582	WP_057080474.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
IEN91_RS13420 (IEN91_13420)	-	2733177..2733746 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
IEN91_RS13425 (IEN91_13425)	yhbY	2733757..2734047 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
IEN91_RS13430 (IEN91_13430)	aroE	2734041..2734883 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22255.41 Da Isoelectric Point: 5.5211

                                >NTDB_id=485604 IEN91_RS13395 WP_015387983.1 2729956..2730570(-) (comEA) [Bacillus velezensis strain FIO1408]

MTKAMMTERMRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAVGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=485604 IEN91_RS13395 WP_015387983.1 2729956..2730570(-) (comEA) [Bacillus velezensis strain FIO1408]

ATGACGAAAGCGATGATGACGGAGCGGATGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCAGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGTCAAACAGCCTCCGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGAGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG

CAGTTGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A6A8LHQ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0485604