Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   IC769_RS01655 Genome accession   NZ_CP061672
Coordinates   351072..351716 (+) Length   214 a.a.
NCBI ID   WP_004705190.1    Uniprot ID   N8R2F5
Organism   Acinetobacter seifertii strain AS23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 346072..356716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC769_RS01635 (IC769_01635) - 346121..346453 (+) 333 WP_004705180.1 hypothetical protein -
  IC769_RS01640 (IC769_01640) - 346456..347279 (+) 824 Protein_317 putative RNA methyltransferase -
  IC769_RS01645 (IC769_01645) ponA 347297..349852 (-) 2556 WP_068932384.1 penicillin-binding protein PBP1a -
  IC769_RS01650 (IC769_01650) pilM 350014..351072 (+) 1059 WP_004705187.1 pilus assembly protein PilM Machinery gene
  IC769_RS01655 (IC769_01655) pilN 351072..351716 (+) 645 WP_004705190.1 PilN domain-containing protein Machinery gene
  IC769_RS01660 (IC769_01660) pilO 351713..352453 (+) 741 WP_191108966.1 type 4a pilus biogenesis protein PilO Machinery gene
  IC769_RS01665 (IC769_01665) pilP 352464..352991 (+) 528 WP_004705193.1 pilus assembly protein PilP Machinery gene
  IC769_RS01670 (IC769_01670) pilQ 353053..355179 (+) 2127 WP_004705194.1 type IV pilus secretin PilQ family protein Machinery gene
  IC769_RS01675 (IC769_01675) aroK 355191..355760 (+) 570 WP_004705195.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 214 a.a.        Molecular weight: 24168.55 Da        Isoelectric Point: 5.3957

>NTDB_id=483995 IC769_RS01655 WP_004705190.1 351072..351716(+) (pilN) [Acinetobacter seifertii strain AS23]
MATINLLPWREELREQRKKQFITLCFGVVVLGITTVFGGWFYFDHKLDDQEQANQLITSTNQNLDQQLKALNGLQEQRDA
IIERMKLIQDLQSQRPVVVRLVDELVRVTPPAMYLTKFSRTGDKFTIEGKAESPNTVAELLRNLEASPWYRNAFMNSFLA
TEDKKDKAASSLLPRVEDHYGSFVVTVDLGEMGMTTADNNADKPAVAKDTGAAK

Nucleotide


Download         Length: 645 bp        

>NTDB_id=483995 IC769_RS01655 WP_004705190.1 351072..351716(+) (pilN) [Acinetobacter seifertii strain AS23]
ATGGCAACAATTAATTTACTACCTTGGCGTGAAGAGCTGAGGGAGCAAAGAAAAAAACAATTTATAACGTTATGCTTTGG
GGTGGTTGTGTTAGGAATTACAACCGTATTTGGGGGGTGGTTCTATTTTGATCATAAATTAGATGATCAAGAACAAGCAA
ATCAACTTATTACAAGTACTAATCAAAACCTTGATCAGCAATTAAAAGCATTAAATGGTTTACAAGAACAACGTGATGCC
ATCATTGAGCGTATGAAATTGATTCAAGATTTACAGAGTCAGCGTCCAGTCGTGGTAAGACTGGTTGATGAGTTAGTCAG
AGTCACGCCGCCTGCAATGTATTTAACGAAATTTAGCCGAACTGGGGATAAATTTACGATTGAAGGTAAAGCTGAAAGTC
CAAATACGGTAGCTGAACTACTCCGTAATCTAGAAGCATCTCCTTGGTATCGAAATGCTTTTATGAACTCTTTCTTGGCT
ACTGAAGATAAAAAAGATAAGGCAGCAAGTTCTCTTCTTCCTAGGGTAGAAGATCATTACGGTAGTTTTGTTGTGACTGT
CGATTTAGGGGAAATGGGCATGACCACAGCTGATAATAATGCTGATAAACCAGCCGTAGCTAAAGATACGGGGGCAGCAA
AATGA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N8R2F5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

92.056

100

0.921
  comN Acinetobacter baylyi ADP1

70.616

98.598

0.696