Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IC773_RS01635 Genome accession   NZ_CP061660
Coordinates   342137..343849 (-) Length   570 a.a.
NCBI ID   WP_077169129.1    Uniprot ID   A0A7H2VJ60
Organism   Acinetobacter seifertii strain AS31     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 337137..348849
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC773_RS01605 (IC773_01600) - 337301..337627 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  IC773_RS01610 (IC773_01605) rlmB 337730..338479 (+) 750 WP_039251214.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  IC773_RS01615 (IC773_01610) - 338514..339428 (+) 915 WP_077169133.1 DMT family transporter -
  IC773_RS01620 (IC773_01615) coaE 339425..340021 (-) 597 WP_077169132.1 dephospho-CoA kinase -
  IC773_RS21250 (IC773_01620) pilD 340023..340883 (-) 861 WP_077169131.1 prepilin peptidase Machinery gene
  IC773_RS01630 (IC773_01625) pilC 340883..342109 (-) 1227 WP_077169130.1 type II secretion system F family protein Machinery gene
  IC773_RS01635 (IC773_01630) pilB 342137..343849 (-) 1713 WP_077169129.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IC773_RS01640 (IC773_01635) tpiA 344139..344933 (+) 795 WP_151731148.1 triose-phosphate isomerase -
  IC773_RS01645 (IC773_01640) secG 344946..345275 (+) 330 WP_019456770.1 preprotein translocase subunit SecG -
  IC773_RS01665 (IC773_01660) rimP 345912..346436 (+) 525 WP_002157009.1 ribosome maturation factor RimP -
  IC773_RS01670 (IC773_01665) nusA 346474..347958 (+) 1485 WP_004699068.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63451.77 Da        Isoelectric Point: 5.2413

>NTDB_id=483883 IC773_RS01635 WP_077169129.1 342137..343849(-) (pilB) [Acinetobacter seifertii strain AS31]
MSALHTSPKFTGFFRRLVEEKHVSAATMQNALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVSLDGPTTQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKTPTDVPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=483883 IC773_RS01635 WP_077169129.1 342137..343849(-) (pilB) [Acinetobacter seifertii strain AS31]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAAATGCATTAGATGCAGCAAAGAGAGCTAAGCAGGATACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTCTTTACTGGCTGAAACAATTTCCGCTGAGTTTGCTGAACCATATTTTGATCTTGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCACTTATTCAAAGAGGCCAAATTTTATATGT
TGCGACCAGCAATCCAAGTAATATTGAAGCGATTGATGCGATTCGTTTTAATAGCAAACTGATGGTTGAGCCTGTCATTG
TTGAGCACCATAAACTCGAAAAAGTTTTAGGTCAGCATTTCGCAGAAGAAAGCAGTTTTGATTTTAATGATGAAGAGTTT
GATCTTGATGTCAGTCTTGATGGCCCCACCACTCAAGAGGATGAAGAAGAAACACCTCAAGGTGATGAAGCTCCCATTGT
TAAATATATTAATAAGTTGCTCATTGATGCCATTCGTATGGGCGCTTCAGACTTACATTTTGAACCGTATGAAAAGTCAT
ATCGGGTACGTTATCGAGTCGACGGTGTGTTACGTCAAATTGCCAATCCACCTTTACAATTGGCAAACCGTTTGGCGTCA
CGTTTAAAAGTAATGTCTCAAATGGATATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATTAAACTTAAGCTCTCAAA
AACTAAAGCAATTGATTTCCGTGTGAACTCACTTCCGACTTTGTTTGGTGAAAAACTAGTTCTACGTATTCTTGATCCAT
CAAGCGCAATGCTAGGGATTGACGCATTAGGATACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAAGGCATGCTTTTAATTACTGGACCAACAGGTTCCGGTAAAACAGTATCTTTATATACAGGCTTAAACATTTTAAATAC
TGAGAGCTCTAATATTTCTACTGCCGAAGACCCTGTAGAAATTAACCTTGAAGGGATTAATCAGGTTAACGTAAATCCCA
AAGTAGGTCTTACGTTTGCTGCTGCACTTAAATCATTTTTACGACAAGACCCTGATATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATCGCAATTAAAGCTGCACAAACGGGTCACATGGTGATGTCAACACTGCATACCAATAGTGC
GCCTGAAACGCTCACTCGCTTACGCAATATGGGGGTTCCTTCCTTCAATATTGCAACATCAGTCAACTTGGTTATTGCTC
AGCGTTTAGCACGCCGCCTTTGCTCTCAATGTAAAACACCTACAGATGTTCCAAAACAAAGTCTATTAGAAATGGGCTTT
ACCGAGCAGGACTTAGCACATCCAGACTTTCGGGTTTTTCAACCTGTGGGTTGTCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGCTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2VJ60

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

98.07

100

0.981
  pilB Acinetobacter baylyi ADP1

80.175

100

0.802
  pilB Legionella pneumophila strain ERS1305867

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

50.178

98.596

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.016

100

0.377
  pilF Thermus thermophilus HB27

37.011

98.596

0.365