Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IC794_RS01530 Genome accession   NZ_CP061568
Coordinates   322145..322885 (+) Length   246 a.a.
NCBI ID   WP_077168923.1    Uniprot ID   A0A7H2VHI1
Organism   Acinetobacter seifertii strain AS71     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 317145..327885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC794_RS01515 (IC794_01515) ponA 317728..320283 (-) 2556 WP_134262504.1 penicillin-binding protein PBP1a -
  IC794_RS01520 (IC794_01520) pilM 320446..321504 (+) 1059 WP_004705187.1 pilus assembly protein PilM Machinery gene
  IC794_RS01525 (IC794_01525) pilN 321504..322148 (+) 645 WP_004705190.1 PilN domain-containing protein Machinery gene
  IC794_RS01530 (IC794_01530) pilO 322145..322885 (+) 741 WP_077168923.1 type 4a pilus biogenesis protein PilO Machinery gene
  IC794_RS01535 (IC794_01535) pilP 322896..323423 (+) 528 WP_077168924.1 pilus assembly protein PilP Machinery gene
  IC794_RS01540 (IC794_01540) pilQ 323485..325611 (+) 2127 WP_151792203.1 type IV pilus secretin PilQ family protein Machinery gene
  IC794_RS01545 (IC794_01545) aroK 325623..326192 (+) 570 WP_004705195.1 shikimate kinase AroK -
  IC794_RS01550 (IC794_01550) aroB 326221..327303 (+) 1083 WP_077168926.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27889.51 Da        Isoelectric Point: 4.8393

>NTDB_id=483232 IC794_RS01530 WP_077168923.1 322145..322885(+) (pilO) [Acinetobacter seifertii strain AS71]
MSQDELQELSLEQTSSKNKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLHAIDNAQAQE
SNLLNEFREKDSKLRNLRQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLENEVKQEVFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPTKDSKSDIPVLNYSIKAKTYRYVEATDTAEQSTQAPTPPASTS
TTAQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=483232 IC794_RS01530 WP_077168923.1 322145..322885(+) (pilO) [Acinetobacter seifertii strain AS71]
ATGAGTCAAGATGAACTTCAAGAATTATCATTAGAGCAAACATCTTCAAAGAACAAGAAATTTAATCTCGATAAATTCCT
TCAACAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCCATATCTGTAAAAATTACTTGTTGGATTTTTATTT
TCTTTGCTGTATTAGCTTTAGGTTATTTTGTTGCTATTCAGCCAAAGCTACACGCAATTGATAATGCTCAAGCTCAGGAA
AGTAACTTGTTAAACGAATTTCGAGAAAAAGATTCGAAATTACGTAACTTGCGGCAGTATCAAGTTCAACTTCAAGAGAT
GCAAGCAAATTTTAATCAGCAATTAGAGCAGTTGCCTAAAGAAACAGAGATTCCTAGTTTGGTTGAGGATATTAACTTAA
CTGGTGTGAACTCAGGATTGAAATTTAAAAATATTCGCTTGGAAAATGAAGTCAAACAGGAAGTCTTTATTGAACAACCG
ATTGCGATAGAAGCCACAGGTGATTATCACGCCTTTGGAGCTTTTGTGAGTAGTATTGCTGCGTTGCCGCGTATCGTAAC
CATGCATGACTTTACTGTTGATGTATCTCCAACTAAAGACAGCAAGTCTGATATTCCTGTATTGAATTATTCTATTAAAG
CGAAAACCTATCGCTATGTAGAAGCAACAGATACAGCGGAGCAATCGACCCAAGCTCCAACACCACCTGCTTCAACTAGC
ACTACTGCTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2VHI1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

91.463

100

0.915
  comO Acinetobacter baylyi ADP1

67.347

99.593

0.671