Detailed information NTG0483230

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: IC794_RS01520
• Genome accession: NZ_CP061568
• Coordinates: 320446..321504 (+)
• Length: 352 a.a.
• NCBI ID: WP_004705187.1
• Uniprot ID: N8R2L8
• Organism: Acinetobacter seifertii strain AS71
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 315446..326504

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IC794_RS01505 (IC794_01505)	-	316551..316883 (+)	333	WP_077168921.1	hypothetical protein	-
IC794_RS01510 (IC794_01510)	-	316886..317710 (+)	825	WP_143221083.1	putative RNA methyltransferase	-
IC794_RS01515 (IC794_01515)	ponA	317728..320283 (-)	2556	WP_134262504.1	penicillin-binding protein PBP1a	-
IC794_RS01520 (IC794_01520)	pilM	320446..321504 (+)	1059	WP_004705187.1	pilus assembly protein PilM	Machinery gene
IC794_RS01525 (IC794_01525)	pilN	321504..322148 (+)	645	WP_004705190.1	PilN domain-containing protein	Machinery gene
IC794_RS01530 (IC794_01530)	pilO	322145..322885 (+)	741	WP_077168923.1	type 4a pilus biogenesis protein PilO	Machinery gene
IC794_RS01535 (IC794_01535)	pilP	322896..323423 (+)	528	WP_077168924.1	pilus assembly protein PilP	Machinery gene
IC794_RS01540 (IC794_01540)	pilQ	323485..325611 (+)	2127	WP_151792203.1	type IV pilus secretin PilQ family protein	Machinery gene
IC794_RS01545 (IC794_01545)	aroK	325623..326192 (+)	570	WP_004705195.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38980.49 Da
• Isoelectric Point: 4.4582

>NTDB_id=483230 IC794_RS01520 WP_004705187.1 320446..321504(+) (pilM) [Acinetobacter seifertii strain AS71]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAINLANPQTKNAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANSNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGVLDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLDE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=483230 IC794_RS01520 WP_004705187.1 320446..321504(+) (pilM) [Acinetobacter seifertii strain AS71]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTTGAAAGCTATGCTTTGATGCCTTTACCTGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTAGCGGAAGCATTGGAAAGGGCGATTAACTTAGCAAATCCCCAAACTAAAAATGCTGCAATT
GCTGTTCCTACATCGACGGTTATTCATAAAACTATCGAGATGGATGCGGATATGAGCGATGATGAACGTGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTGGATGAGGTGAGCCTAGATTTTGAGGTTCTACCTGACCGACTCG
CGAATTCAAATCGTGTAAATGTACTCTTAGTCGCAACAAGAACGGAAAACGTAGAAACGCGCGTTGAGGTTCTTGAGTTG
GCAGATTTAAATCCTAAATTGGCTGATGTTGAAAGTTATGCTGTTGAGCGCGCATTTAGTGTGTTTGCTGATAGCTTACC
TATGGGTGCAAATACGATAGGGGTTTTAGATATTGGCCATACCATGACAACATTGTCTGTCATGCAAAACGGCAAAATTA
TTTATACCCGTGAGCAAGTTTTTGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGACGAA
GCAAGTCGAGCGAAAAAAGATCGTTCTTTACCGGATGACTATGAAATTGAAGTATTAGATCCGTTTCTAGATGCCGTAGT
TCAGCAAGCGGCTCGCTCTTTACAATTCTTCTTTTCTTCATCTCAGTTTAACGAAATAGACCATATTCTGCTTGCAGGCG
GAAATGCGAATATTCCAGGTCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACTATTGCCAATCCATTTTTA
CAGATGGGTTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCCTCTTCTTTAATGGTGGCGTGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N8R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	98.295	100	0.983
comM	Acinetobacter nosocomialis M2	98.011	100	0.98
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.373	100	0.426