Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   IC796_RS01675 Genome accession   NZ_CP061561
Coordinates   352194..352934 (+) Length   246 a.a.
NCBI ID   WP_151682782.1    Uniprot ID   A0A7H2QM36
Organism   Acinetobacter seifertii strain AS73     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 347194..357934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IC796_RS01660 (IC796_01660) ponA 347778..350333 (-) 2556 WP_039251098.1 penicillin-binding protein PBP1a -
  IC796_RS01665 (IC796_01665) pilM 350495..351553 (+) 1059 WP_191036737.1 pilus assembly protein PilM Machinery gene
  IC796_RS01670 (IC796_01670) pilN 351553..352197 (+) 645 WP_151682783.1 PilN domain-containing protein Machinery gene
  IC796_RS01675 (IC796_01675) pilO 352194..352934 (+) 741 WP_151682782.1 type 4a pilus biogenesis protein PilO Machinery gene
  IC796_RS01680 (IC796_01680) pilP 352945..353472 (+) 528 WP_151682781.1 pilus assembly protein PilP Machinery gene
  IC796_RS01685 (IC796_01685) pilQ 353534..355645 (+) 2112 WP_151682780.1 type IV pilus secretin PilQ family protein Machinery gene
  IC796_RS01690 (IC796_01690) aroK 355657..356226 (+) 570 WP_151682779.1 shikimate kinase AroK -
  IC796_RS01695 (IC796_01695) aroB 356255..357253 (+) 999 WP_223850132.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 28042.78 Da        Isoelectric Point: 4.9865

>NTDB_id=483193 IC796_RS01675 WP_151682782.1 352194..352934(+) (pilO) [Acinetobacter seifertii strain AS73]
MSQDELHELSLEQSVSKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLENEVKQEVFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDSKSDIPVLNYSIKAKTYRYVEATDRVEQSPQALKTPSSNS
TTDQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=483193 IC796_RS01675 WP_151682782.1 352194..352934(+) (pilO) [Acinetobacter seifertii strain AS73]
ATGAGTCAAGATGAACTTCACGAGTTATCATTAGAGCAATCGGTTTCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAACAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCTATATCTGTAAAAATTACTTGTTGGATTTTTATTT
TCTTTGCTGTATTAGCTTTGGGTTATTTTGTTGCTATTCAGCCAAAGCTACAAGCAATTGATAATGCTCAAGCTCAGGAA
AGTAACTTGTTAAACGAATTTCGAGAAAAAGATTCGAAATTACGTAACTTGCAGCAGTATCAAGTCCAACTTCAAGAGAT
GCAAGCAAATTTTAATCAGCAATTAGAGCAGTTGCCTAAAGAAACAGAGATTCCTAGTTTAGTTGAAGATATCAATTTAA
CGGGTGTGAACTCAGGATTGAAATTTAAAAATATTCGCTTGGAAAATGAAGTAAAACAGGAAGTCTTTATTGAACAACCG
ATTGCGATAGAAGCTACAGGTGATTATCACGCTTTTGGAGCTTTTGTGAGTAGTATTGCTGCGTTGCCGCGTATCGTAAC
CATGCACGACTTTACTGTTGATGTATCTCCAGCGAAAGACAGCAAGTCTGATATTCCTGTATTGAACTATTCTATTAAAG
CGAAAACCTATCGTTATGTAGAAGCAACAGATAGAGTAGAGCAGTCGCCCCAAGCTTTAAAAACACCTTCTTCAAATAGC
ACGACTGATCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H2QM36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

90.65

100

0.907
  comO Acinetobacter baylyi ADP1

68.033

99.187

0.675