Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilX   Type   Machinery gene
Locus tag   NMBNZ0533_RS05290 Genome accession   NC_017518
Coordinates   991349..991822 (+) Length   157 a.a.
NCBI ID   WP_014581124.1    Uniprot ID   -
Organism   Neisseria meningitidis NZ-05/33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 989160..1003766 991349..991822 within 0


Gene organization within MGE regions


Location: 989160..1003766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMBNZ0533_RS05275 (NMBNZ0533_0937) pilV 989193..989807 (+) 615 WP_014581127.1 type IV pilus modification protein PilV Machinery gene
  NMBNZ0533_RS05280 (NMBNZ0533_0938) pilJ 989804..990772 (+) 969 WP_014582408.1 PilW family protein Machinery gene
  NMBNZ0533_RS05285 (NMBNZ0533_0939) pilK 990751..991344 (+) 594 WP_014581125.1 pilus assembly protein Machinery gene
  NMBNZ0533_RS05290 (NMBNZ0533_0940) pilX 991349..991822 (+) 474 WP_014581124.1 PilX family type IV pilin Machinery gene
  NMBNZ0533_RS05295 (NMBNZ0533_0941) dut 992385..992837 (+) 453 WP_002229326.1 dUTP diphosphatase -
  NMBNZ0533_RS05300 (NMBNZ0533_0942) dapC 992914..994101 (+) 1188 WP_014582409.1 succinyldiaminopimelate transaminase -
  NMBNZ0533_RS05305 (NMBNZ0533_0943) yaaA 994265..995044 (+) 780 WP_002235690.1 peroxide stress protein YaaA -
  NMBNZ0533_RS05320 (NMBNZ0533_0946) - 995601..996797 (+) 1197 WP_002223440.1 integrase arm-type DNA-binding domain-containing protein -
  NMBNZ0533_RS05325 (NMBNZ0533_0947) - 996954..997334 (+) 381 WP_002220683.1 type II toxin-antitoxin system PemK/MazF family toxin -
  NMBNZ0533_RS05340 (NMBNZ0533_0949) - 997882..998238 (+) 357 WP_002223441.1 DUF4760 domain-containing protein -
  NMBNZ0533_RS05345 (NMBNZ0533_0950) - 999107..1000024 (+) 918 WP_002219437.1 KilA-N domain-containing protein -
  NMBNZ0533_RS05350 (NMBNZ0533_0951) - 1000082..1000291 (-) 210 WP_002220687.1 hypothetical protein -
  NMBNZ0533_RS05355 (NMBNZ0533_0952) - 1000288..1000428 (-) 141 WP_002223442.1 hypothetical protein -
  NMBNZ0533_RS05360 (NMBNZ0533_0953) - 1000428..1000619 (-) 192 WP_002219435.1 type ISP restriction/modification enzyme -
  NMBNZ0533_RS05365 (NMBNZ0533_0954) - 1000622..1000915 (-) 294 WP_002220689.1 hypothetical protein -
  NMBNZ0533_RS05370 (NMBNZ0533_0955) - 1000912..1001172 (-) 261 WP_002223443.1 hypothetical protein -
  NMBNZ0533_RS05375 (NMBNZ0533_0956) - 1001207..1001422 (-) 216 WP_002219434.1 hypothetical protein -
  NMBNZ0533_RS05380 (NMBNZ0533_0957) - 1001492..1002346 (-) 855 WP_002219433.1 YfdQ family protein -
  NMBNZ0533_RS05385 (NMBNZ0533_0958) - 1002371..1002730 (-) 360 WP_002219432.1 hypothetical protein -
  NMBNZ0533_RS05390 (NMBNZ0533_0959) - 1002798..1002998 (-) 201 WP_002219431.1 hypothetical protein -
  NMBNZ0533_RS05395 (NMBNZ0533_0960) - 1003242..1003667 (-) 426 WP_002219430.1 hypothetical protein -

Sequence

Protein


Download         Length: 157 a.a.        Molecular weight: 17529.28 Da        Isoelectric Point: 9.4429

>NTDB_id=48155 NMBNZ0533_RS05290 WP_014581124.1 991349..991822(+) (pilX) [Neisseria meningitidis NZ-05/33]
MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIERGYQSQLYTEMVGINNISKQFILKSPLDDNQTIKSKLEIFVSGYKM
NPKIAEKYNVSVHFVNKEKPRAYSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASARAHLETLSSDVGCEAFSNRKK

Nucleotide


Download         Length: 474 bp        

>NTDB_id=48155 NMBNZ0533_RS05290 WP_014581124.1 991349..991822(+) (pilX) [Neisseria meningitidis NZ-05/33]
ATGGAACAAAAAGGGTTTACATTGATTGAGATGATGATAGTCGTCGCGATACTCGGCATCATCAGCGTCATTGCCATACC
TTCTTATCAGAGTTATATTGAAAGAGGCTATCAGTCCCAGCTTTATACGGAGATGGTCGGTATCAACAATATTTCCAAAC
AGTTTATTTTGAAAAGTCCCCTGGACGATAATCAGACCATCAAGAGCAAACTGGAAATATTTGTCTCAGGCTATAAGATG
AATCCGAAAATTGCCGAAAAATATAATGTTTCGGTGCATTTTGTCAATAAGGAAAAACCAAGGGCATACAGCTTGGTCGG
CGTTCCAAAGACGGGGACGGGTTATACTTTGTCGGTATGGATGAACAGCGTGGGCGACGGATACAAATGCCGTGATGCCG
CTTCTGCCCGAGCCCATTTGGAGACCTTGTCCTCAGATGTCGGCTGTGAAGCCTTCTCTAATCGTAAAAAATAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-25)

Minor type IV pilin, PilX (Pilin_PilX)

(31-157)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilX Neisseria meningitidis 8013

98.089

100

0.981
  pilL Neisseria gonorrhoeae MS11

85.35

100

0.854

Multiple sequence alignment