Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   IBL27_RS00975 Genome accession   NZ_CP061071
Coordinates   203841..204872 (+) Length   343 a.a.
NCBI ID   WP_255264529.1    Uniprot ID   -
Organism   Streptococcus mutans B04Sm5     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 198841..209872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IBL27_RS00965 (IBL27_00965) - 202449..202832 (+) 384 WP_002263434.1 DUF1033 family protein -
  IBL27_RS00970 (IBL27_00970) comYA 202964..203905 (+) 942 WP_002263435.1 competence type IV pilus ATPase ComGA Machinery gene
  IBL27_RS00975 (IBL27_00975) comYB 203841..204872 (+) 1032 WP_255264529.1 competence type IV pilus assembly protein ComGB Machinery gene
  IBL27_RS00980 (IBL27_00980) comYC 204872..205186 (+) 315 WP_002279331.1 competence type IV pilus major pilin ComGC Machinery gene
  IBL27_RS00985 (IBL27_00985) comYD 205173..205577 (+) 405 WP_002279330.1 competence type IV pilus minor pilin ComGD Machinery gene
  IBL27_RS00990 (IBL27_00990) comYE 205549..205842 (+) 294 WP_002263439.1 competence type IV pilus minor pilin ComGE Machinery gene
  IBL27_RS00995 (IBL27_00995) comYF 205829..206263 (+) 435 WP_002264245.1 competence type IV pilus minor pilin ComGF Machinery gene
  IBL27_RS01000 (IBL27_01000) comYG 206241..206630 (+) 390 WP_002263441.1 competence type IV pilus minor pilin ComGG Machinery gene
  IBL27_RS01005 (IBL27_01005) comYH 206685..207638 (+) 954 WP_002309243.1 class I SAM-dependent methyltransferase Machinery gene
  IBL27_RS01010 (IBL27_01010) - 207697..208896 (+) 1200 WP_002265895.1 acetate kinase -
  IBL27_RS01015 (IBL27_01015) - 209449..209694 (+) 246 WP_002265894.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 39816.44 Da        Isoelectric Point: 9.5097

>NTDB_id=480216 IBL27_RS00975 WP_255264529.1 203841..204872(+) (comYB) [Streptococcus mutans B04Sm5]
MTFLQQDISLKEKHKQKKLKIKKQRKVIQLFNNLFTSGFNLTEIIDFLERSHLLAEIYTKKMRQGLLNGLHLAEIMSSLG
FSDDVVTQLALSEVHGNTQKSLLKIESYLNNLILVKKKLVEVITYPIILLIFLVVIMLGLKNYLLPQLEKGNFATRLISN
FPILFLLGCCFFILLVGIFTFFSRKISRLHLIGFCSHLPFAGKYLQLYLTAYYAREWGNLIGQGVEMAQIVRLMQDQKSQ
LFREIGYDMEKALLSGQEFHQKVLDYPFFLKELSLMIEYGEVKSKLGNELEIYAEETWEHFFAKLKKATQFVQPLIFVFV
AIVIVMIYAAMLLPIYQNMEVNL

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=480216 IBL27_RS00975 WP_255264529.1 203841..204872(+) (comYB) [Streptococcus mutans B04Sm5]
ATGACCTTTTTACAGCAGGATATATCACTGAAGGAGAAGCACAAGCAGAAGAAATTGAAGATTAAAAAGCAAAGAAAAGT
TATCCAGCTTTTTAATAATCTCTTTACTAGTGGTTTTAATTTAACAGAAATTATAGATTTTTTAGAACGAAGTCACTTGT
TAGCAGAAATATACACAAAGAAAATGCGACAAGGGTTGTTGAATGGTCTGCATTTGGCTGAAATAATGAGTTCTTTGGGA
TTCTCAGATGATGTTGTTACTCAATTAGCACTTTCTGAAGTTCATGGAAACACTCAAAAAAGTCTTTTAAAGATTGAATC
TTATCTAAATAATCTGATACTTGTTAAAAAGAAATTAGTTGAGGTTATCACTTATCCGATCATTTTATTGATTTTTCTTG
TCGTTATTATGTTGGGATTAAAGAATTATTTATTACCACAACTTGAAAAGGGAAATTTTGCGACTCGGTTGATTTCTAAT
TTCCCAATACTTTTCTTGCTGGGATGTTGCTTTTTTATTTTACTAGTTGGCATTTTTACTTTCTTTTCACGAAAGATCAG
TCGTCTGCATTTAATTGGTTTTTGCAGCCATTTGCCTTTTGCTGGTAAGTATTTGCAACTTTATTTAACAGCTTATTATG
CGCGTGAATGGGGTAATCTTATTGGGCAAGGAGTAGAAATGGCTCAGATAGTTAGATTGATGCAAGATCAAAAGTCACAA
CTTTTTAGAGAAATTGGCTATGATATGGAGAAGGCTCTGCTATCAGGTCAAGAGTTTCATCAAAAAGTTTTAGATTATCC
TTTCTTTTTAAAAGAACTCAGCCTTATGATTGAATATGGAGAAGTAAAGTCAAAATTGGGAAATGAATTGGAAATTTATG
CTGAGGAAACATGGGAACACTTTTTTGCAAAGTTAAAAAAAGCAACACAGTTTGTTCAGCCACTCATCTTTGTTTTTGTA
GCTATTGTTATTGTTATGATTTATGCAGCCATGCTTTTGCCAATTTATCAGAATATGGAGGTTAATTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(29-146)

Type II secretion system (T2SS), protein F (T2SSF)

(214-334)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA159

99.708

100

0.997
  comYB Streptococcus mutans UA140

99.708

100

0.997
  comYB Streptococcus gordonii str. Challis substr. CH1

60.058

100

0.601
  comGB/cglB Streptococcus mitis NCTC 12261

58.407

98.834

0.577
  comGB/cglB Streptococcus mitis SK321

56.548

97.959

0.554
  comGB/cglB Streptococcus pneumoniae Rx1

55.952

97.959

0.548
  comGB/cglB Streptococcus pneumoniae D39

55.952

97.959

0.548
  comGB/cglB Streptococcus pneumoniae R6

55.952

97.959

0.548
  comGB/cglB Streptococcus pneumoniae TIGR4

55.952

97.959

0.548
  comGB Lactococcus lactis subsp. cremoris KW2

51.929

98.251

0.51