Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   J5V74_RS04090 Genome accession   NZ_CP071947
Coordinates   905959..906582 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 2020HL-00861     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 900959..911582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5V74_RS04070 (J5V74_04070) aroB 901554..902660 (-) 1107 WP_003162482.1 3-dehydroquinate synthase -
  J5V74_RS04075 (J5V74_04075) aroK 902710..903228 (-) 519 WP_003095833.1 shikimate kinase AroK -
  J5V74_RS04080 (J5V74_04080) pilQ 903240..905384 (-) 2145 WP_014603377.1 type 4a pilus secretin PilQ Machinery gene
  J5V74_RS04085 (J5V74_04085) comL/pilP 905438..905962 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  J5V74_RS04090 (J5V74_04090) pilO 905959..906582 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  J5V74_RS04095 (J5V74_04095) pilN 906579..907175 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  J5V74_RS04100 (J5V74_04100) pilM 907175..908239 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  J5V74_RS04105 (J5V74_04105) - 908424..910892 (+) 2469 WP_003114576.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=479742 J5V74_RS04090 WP_003103268.1 905959..906582(-) (pilO) [Pseudomonas aeruginosa strain 2020HL-00861]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=479742 J5V74_RS04090 WP_003103268.1 905959..906582(-) (pilO) [Pseudomonas aeruginosa strain 2020HL-00861]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAGCAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401