Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   J3K87_RS04885 Genome accession   NZ_CP071698
Coordinates   1064954..1065400 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   Q8VU03
Organism   Actinobacillus pleuropneumoniae strain SC1810     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1059954..1070400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J3K87_RS04860 (J3K87_04830) yacG 1060861..1061049 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  J3K87_RS04865 (J3K87_04835) coaE 1061031..1061669 (-) 639 WP_237593619.1 dephospho-CoA kinase -
  J3K87_RS04870 (J3K87_04840) - 1061691..1062329 (-) 639 WP_005604550.1 prepilin peptidase -
  J3K87_RS04875 (J3K87_04845) pilC 1062356..1063558 (-) 1203 WP_012263006.1 type II secretion system F family protein Machinery gene
  J3K87_RS04880 (J3K87_04850) pilB 1063542..1064936 (-) 1395 WP_237593620.1 GspE/PulE family protein Machinery gene
  J3K87_RS04885 (J3K87_04855) pilA 1064954..1065400 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  J3K87_RS04890 (J3K87_04860) radA/sms 1065582..1066961 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  J3K87_RS04895 (J3K87_04865) - 1067005..1067238 (-) 234 WP_237593621.1 YgjV family protein -
  J3K87_RS04900 (J3K87_04870) folA 1067368..1067856 (-) 489 WP_252590656.1 type 3 dihydrofolate reductase -
  J3K87_RS04905 (J3K87_04875) proB 1067943..1069034 (+) 1092 WP_237593623.1 glutamate 5-kinase -
  J3K87_RS04910 (J3K87_04880) - 1069047..1069823 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=478459 J3K87_RS04885 WP_005597397.1 1064954..1065400(-) (pilA) [Actinobacillus pleuropneumoniae strain SC1810]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=478459 J3K87_RS04885 WP_005597397.1 1064954..1065400(-) (pilA) [Actinobacillus pleuropneumoniae strain SC1810]
ATGCAAAAATTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8VU03

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527