Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   H7788_RS00685 Genome accession   NZ_CP060647
Coordinates   108249..108683 (+) Length   144 a.a.
NCBI ID   WP_002986542.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain TSPY136     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 103249..113683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7788_RS00655 (H7788_00655) - 104917..105282 (+) 366 WP_002986560.1 DUF1033 family protein -
  H7788_RS00660 (H7788_00660) comYA 105376..106314 (+) 939 WP_030127399.1 competence type IV pilus ATPase ComGA Machinery gene
  H7788_RS00665 (H7788_00665) comYB 106250..107284 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  H7788_RS00670 (H7788_00670) comYC 107286..107612 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  H7788_RS00675 (H7788_00675) comGD 107587..108015 (+) 429 WP_030127397.1 competence type IV pilus minor pilin ComGD -
  H7788_RS00680 (H7788_00680) comGE 107972..108256 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  H7788_RS00685 (H7788_00685) comYF 108249..108683 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  H7788_RS00690 (H7788_00690) comGG 108667..108993 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  H7788_RS00695 (H7788_00695) comYH 109091..110044 (+) 954 WP_030127396.1 class I SAM-dependent methyltransferase Machinery gene
  H7788_RS00700 (H7788_00700) - 110103..111299 (+) 1197 WP_011888536.1 acetate kinase -
  H7788_RS00705 (H7788_00705) - 111486..111794 (+) 309 WP_030126458.1 hypothetical protein -
  H7788_RS00710 (H7788_00710) proC 111877..112647 (-) 771 WP_228614917.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16573.20 Da        Isoelectric Point: 10.3584

>NTDB_id=477395 H7788_RS00685 WP_002986542.1 108249..108683(+) (comYF) [Streptococcus pyogenes strain TSPY136]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=477395 H7788_RS00685 WP_002986542.1 108249..108683(+) (comYF) [Streptococcus pyogenes strain TSPY136]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

45.985

95.139

0.437
  comGF/cglF Streptococcus mitis NCTC 12261

44.03

93.056

0.41
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396