Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   J1F19_RS09845 Genome accession   NZ_CP071439
Coordinates   2073770..2074429 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain EcPNK004     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2068770..2079429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J1F19_RS09825 (J1F19_09825) plsC 2068916..2069653 (-) 738 WP_000965711.1 1-acylglycerol-3-phosphate O-acyltransferase -
  J1F19_RS09830 (J1F19_09830) parC 2069887..2072145 (-) 2259 WP_001586259.1 DNA topoisomerase IV subunit A -
  J1F19_RS09835 (J1F19_09835) ygiV 2072691..2073173 (-) 483 WP_000183504.1 GyrI-like domain-containing protein -
  J1F19_RS09840 (J1F19_09840) ygiW 2073226..2073618 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  J1F19_RS09845 (J1F19_09845) ciaR 2073770..2074429 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  J1F19_RS09850 (J1F19_09850) qseC 2074426..2075775 (+) 1350 WP_000673370.1 quorum sensing histidine kinase QseC -
  J1F19_RS09855 (J1F19_09855) mdaB 2075885..2076466 (+) 582 WP_000072420.1 NADPH:quinone oxidoreductase MdaB -
  J1F19_RS09860 (J1F19_09860) ygiN 2076497..2076811 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  J1F19_RS09865 (J1F19_09865) - 2076856..2077743 (-) 888 WP_000614957.1 MurR/RpiR family transcriptional regulator -
  J1F19_RS09870 (J1F19_09870) - 2077740..2078687 (-) 948 WP_001315018.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=476917 J1F19_RS09845 WP_001221493.1 2073770..2074429(+) (ciaR) [Escherichia coli strain EcPNK004]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=476917 J1F19_RS09845 WP_001221493.1 2073770..2074429(+) (ciaR) [Escherichia coli strain EcPNK004]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTAGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACTCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365