Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   J0G23_RS10720 Genome accession   NZ_CP071263
Coordinates   2181129..2181788 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain MEI003     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2176129..2186788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0G23_RS10705 (J0G23_10635) ygiS 2178334..2179941 (-) 1608 WP_001327858.1 ABC transporter substrate-binding protein -
  J0G23_RS10710 (J0G23_10640) ygiV 2180050..2180532 (-) 483 WP_024229347.1 GyrI-like domain-containing protein -
  J0G23_RS10715 (J0G23_10645) ygiW 2180585..2180977 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  J0G23_RS10720 (J0G23_10650) ciaR 2181129..2181788 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  J0G23_RS10725 (J0G23_10655) qseC 2181785..2183134 (+) 1350 WP_001544946.1 quorum sensing histidine kinase QseC -
  J0G23_RS10730 (J0G23_10660) mdaB 2183244..2183825 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  J0G23_RS10735 (J0G23_10665) ygiN 2183856..2184170 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  J0G23_RS10740 (J0G23_10670) - 2184215..2185102 (-) 888 WP_000614957.1 MurR/RpiR family transcriptional regulator -
  J0G23_RS10745 (J0G23_10675) - 2185099..2186046 (-) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=476437 J0G23_RS10720 WP_001221493.1 2181129..2181788(+) (ciaR) [Escherichia coli strain MEI003]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=476437 J0G23_RS10720 WP_001221493.1 2181129..2181788(+) (ciaR) [Escherichia coli strain MEI003]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTCGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGTGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365