Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   JS609_RS13750 Genome accession   NZ_CP071043
Coordinates   2660687..2661691 (-) Length   334 a.a.
NCBI ID   WP_003229718.1    Uniprot ID   O32055
Organism   Bacillus subtilis isolate ELA2001105     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2655687..2666691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JS609_RS13720 (JS609_02696) yrbG 2656661..2657317 (-) 657 WP_151262358.1 DUF421 domain-containing protein -
  JS609_RS13725 (JS609_02697) - 2657515..2657904 (+) 390 WP_004398667.1 TIGR04086 family membrane protein -
  JS609_RS13730 (JS609_02698) yajC 2657961..2658227 (-) 267 WP_004398708.1 preprotein translocase subunit YajC -
  JS609_RS13735 (JS609_02699) tgt 2658264..2659409 (-) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  JS609_RS13740 (JS609_02700) queA 2659436..2660464 (-) 1029 WP_003229723.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  JS609_RS13745 (JS609_02701) yrzS 2660494..2660694 (-) 201 WP_003222669.1 DUF2905 domain-containing protein -
  JS609_RS13750 (JS609_02702) ruvB 2660687..2661691 (-) 1005 WP_003229718.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  JS609_RS13755 (JS609_02703) ruvA 2661702..2662307 (-) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  JS609_RS13760 (JS609_02704) bofC 2662446..2662958 (-) 513 WP_003229715.1 sporulation cell-cell signaling protein BofC -
  JS609_RS13765 (JS609_02705) csbX 2663006..2664313 (-) 1308 WP_003246197.1 MFS transporter -
  JS609_RS13770 (JS609_02706) iolG 2664384..2665408 (-) 1025 Protein_2672 inositol 2-dehydrogenase -
  JS609_RS13775 (JS609_02707) yrzF 2665646..2666293 (+) 648 WP_003246159.1 phosphotransferase -
  JS609_RS13780 (JS609_02708) - 2666339..2666461 (-) 123 WP_003229707.1 hypothetical protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37467.78 Da        Isoelectric Point: 5.1631

>NTDB_id=475861 JS609_RS13750 WP_003229718.1 2660687..2661691(-) (ruvB) [Bacillus subtilis isolate ELA2001105]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=475861 JS609_RS13750 WP_003229718.1 2660687..2661691(-) (ruvB) [Bacillus subtilis isolate ELA2001105]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGCCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTCATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT
ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518