Detailed information NTG0474381

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: H7F25_RS15065
• Genome accession: NZ_CP060191
• Coordinates: 2961268..2962665 (-)
• Length: 465 a.a.
• NCBI ID: WP_142927723.1
• Uniprot ID: -
• Organism: Bacillus sp. PAMC28571
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2956268..2967665

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7F25_RS15030 (H7F25_15030)	flgL	2956468..2957376 (-)	909	WP_024423617.1	flagellar hook-associated protein FlgL	-
H7F25_RS15035 (H7F25_15035)	flgK	2957386..2958906 (-)	1521	WP_024423618.1	flagellar hook-associated protein FlgK	-
H7F25_RS15040 (H7F25_15040)	-	2958922..2959404 (-)	483	WP_034623751.1	flagellar protein FlgN	-
H7F25_RS15045 (H7F25_15045)	flgM	2959423..2959686 (-)	264	WP_024423620.1	flagellar biosynthesis anti-sigma factor FlgM	-
H7F25_RS15050 (H7F25_15050)	-	2959766..2960185 (-)	420	WP_024423621.1	TIGR03826 family flagellar region protein	-
H7F25_RS15055 (H7F25_15055)	comFC	2960243..2960899 (-)	657	WP_253207496.1	ComF family protein	Machinery gene
H7F25_RS15060 (H7F25_15060)	-	2960926..2961219 (-)	294	WP_024423623.1	late competence development ComFB family protein	-
H7F25_RS15065 (H7F25_15065)	comFA	2961268..2962665 (-)	1398	WP_142927723.1	DEAD/DEAH box helicase	Machinery gene
H7F25_RS15070 (H7F25_15070)	-	2962786..2963628 (-)	843	WP_024423625.1	DegV family protein	-
H7F25_RS15075 (H7F25_15075)	degU	2963844..2964533 (-)	690	WP_008348273.1	two-component system response regulator DegU	Regulator
H7F25_RS15080 (H7F25_15080)	degS	2964598..2965773 (-)	1176	WP_003213166.1	sensor histidine kinase	Regulator
H7F25_RS15085 (H7F25_15085)	-	2965994..2966662 (+)	669	WP_012011434.1	YigZ family protein	-

Sequence

Protein

• Length: 465 a.a.
• Molecular weight: 53208.16 Da
• Isoelectric Point: 10.0241

>NTDB_id=474381 H7F25_RS15065 WP_142927723.1 2961268..2962665(-) (comFA) [Bacillus sp. PAMC28571]
MNMDKAMELMRQLHSRHLLTVETKCPQSNLDWLEEKGLVNRSPAIERKANGFTCCRCGVSHKRYFAHSPCEVCQKGCVYC
RSCIMMGKAAECGFLYEWTGPQMVETCRAELTWQGELSKGQKRASERMIEAIKNKFDLLVWAVCGAGKTEVLFHGIEYAL
NQGMRVCIATPRTDVVLELEPRLRKAFQGVTITVLYGGSSQRFQITPLVITTTHQLMRYKNAFDVLIVDEVDAFPYSMDE
RLQFAVLKAMRKNRGVRIYLSATPSKKMTREVSRGKLEAIKIPLRFHEQPLPVPTFHWIGHWKKKLKKNQLPPKVMNWMQ
RHIANRRRILLFVPSIATMKKVTKILREQSLNVEGVSADDPDRKQKVQHFRDYVYDVLVTTTILERGVTIPNVQVGVLGS
ESTIFTESALVQISGRVGRHPDYCTGDVFLFHFGLTRSMKQAKKHIAKMNDTAAKEFSEKQCGFN

Nucleotide

• Length: 1398 bp

>NTDB_id=474381 H7F25_RS15065 WP_142927723.1 2961268..2962665(-) (comFA) [Bacillus sp. PAMC28571]
TTGAATATGGACAAAGCAATGGAACTAATGCGGCAGCTGCATTCACGCCATCTGCTCACCGTTGAAACAAAGTGCCCACA
GTCCAATTTAGATTGGCTGGAGGAGAAGGGCTTAGTCAATCGGTCTCCAGCCATTGAGAGGAAAGCAAATGGTTTTACAT
GCTGCCGGTGCGGTGTATCACACAAGCGGTATTTCGCTCATTCTCCATGTGAAGTGTGTCAAAAGGGTTGTGTGTACTGT
AGATCATGCATCATGATGGGAAAAGCAGCAGAATGTGGGTTTCTTTATGAATGGACAGGTCCACAAATGGTAGAAACATG
TCGAGCCGAATTAACATGGCAGGGAGAGCTGTCTAAAGGACAAAAAAGAGCGTCAGAAAGAATGATTGAAGCTATTAAAA
ACAAATTTGATCTACTTGTTTGGGCGGTTTGCGGAGCAGGGAAAACGGAAGTGCTTTTTCACGGAATCGAATATGCGTTA
AATCAAGGAATGAGGGTCTGTATTGCGACACCTAGAACAGATGTAGTGCTTGAACTCGAACCACGGCTTAGAAAAGCATT
TCAAGGGGTGACAATTACCGTACTTTACGGAGGCAGTTCTCAAAGGTTTCAAATAACACCGCTTGTGATCACCACAACCC
ACCAGCTGATGAGATACAAAAATGCTTTTGATGTGCTCATTGTAGATGAAGTCGATGCCTTCCCTTATTCAATGGATGAG
CGTCTCCAATTTGCTGTTCTAAAGGCGATGAGAAAGAACAGAGGGGTTAGGATTTATTTAAGTGCGACACCCTCTAAAAA
AATGACGAGAGAGGTTTCTCGTGGAAAACTGGAAGCGATAAAAATTCCTTTGCGTTTTCACGAGCAACCATTACCCGTAC
CGACCTTTCATTGGATCGGTCATTGGAAAAAGAAATTAAAAAAGAACCAGCTGCCCCCTAAAGTGATGAATTGGATGCAG
AGACATATTGCAAATAGGAGAAGAATATTACTTTTTGTTCCTTCAATTGCTACCATGAAGAAGGTAACGAAGATTCTTCG
AGAACAATCCTTAAATGTGGAGGGAGTATCTGCCGATGATCCGGATAGGAAACAAAAGGTTCAGCACTTTAGAGATTACG
TATACGATGTACTAGTTACAACCACTATTCTAGAAAGAGGCGTAACGATTCCAAATGTTCAAGTGGGGGTACTAGGTTCG
GAATCTACTATTTTTACAGAGAGTGCACTTGTTCAGATATCTGGACGGGTAGGCAGACACCCGGATTATTGTACAGGAGA
TGTTTTCCTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGCAAAAATGAATGATACGGCTG
CAAAAGAGTTTTCTGAAAAACAGTGTGGGTTCAACTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (116-265)

• Helicase conserved C-terminal domain (Helicase_C) (324-420)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	52.412	98.065	0.514
comFA/cflA	Streptococcus pneumoniae Rx1	38.497	94.409	0.363
comFA/cflA	Streptococcus pneumoniae D39	38.497	94.409	0.363
comFA/cflA	Streptococcus pneumoniae R6	38.497	94.409	0.363
comFA/cflA	Streptococcus pneumoniae TIGR4	38.269	94.409	0.361