Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   JT749_RS04885 Genome accession   NZ_CP070467
Coordinates   1069406..1070029 (+) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain B17416     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1064406..1075029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JT749_RS04870 (JT749_04870) - 1065093..1067564 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  JT749_RS04875 (JT749_04875) pilM 1067749..1068813 (+) 1065 WP_108720490.1 type IV pilus assembly protein PilM Machinery gene
  JT749_RS04880 (JT749_04880) pilN 1068813..1069409 (+) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  JT749_RS04885 (JT749_04885) pilO 1069406..1070029 (+) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  JT749_RS04890 (JT749_04890) comL/pilP 1070026..1070550 (+) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  JT749_RS04895 (JT749_04895) pilQ 1070604..1072736 (+) 2133 WP_033989912.1 type 4a pilus secretin PilQ Machinery gene
  JT749_RS04900 (JT749_04900) aroK 1072748..1073266 (+) 519 WP_003095833.1 shikimate kinase AroK -
  JT749_RS04905 (JT749_04905) aroB 1073316..1074422 (+) 1107 WP_033989914.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=474209 JT749_RS04885 WP_003116135.1 1069406..1070029(+) (pilO) [Pseudomonas aeruginosa strain B17416]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=474209 JT749_RS04885 WP_003116135.1 1069406..1070029(+) (pilO) [Pseudomonas aeruginosa strain B17416]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCTTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396