Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   JTF63_RS03420 Genome accession   NZ_CP070232
Coordinates   715524..716183 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain 10R     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 710524..721183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JTF63_RS03395 (JTF63_03395) parE 710615..712507 (+) 1893 WP_001586263.1 DNA topoisomerase IV subunit B -
  JTF63_RS03400 (JTF63_03400) ygiN 712555..712869 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  JTF63_RS03405 (JTF63_03405) mdaB 712900..713481 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  JTF63_RS03410 (JTF63_03410) ygiZ 713800..714132 (+) 333 WP_000917685.1 DUF2645 family protein -
  JTF63_RS03415 (JTF63_03415) qseC 714178..715527 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  JTF63_RS03420 (JTF63_03420) ciaR 715524..716183 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  JTF63_RS03425 (JTF63_03425) ygiW 716335..716727 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  JTF63_RS03430 (JTF63_03430) ygiV 716780..717262 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  JTF63_RS03435 (JTF63_03435) mqsR 717467..717763 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  JTF63_RS03440 (JTF63_03440) mqsA 717765..718160 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  JTF63_RS03445 (JTF63_03445) ygiS 718293..719900 (+) 1608 WP_001295629.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=473702 JTF63_RS03420 WP_001221493.1 715524..716183(-) (ciaR) [Escherichia coli strain 10R]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=473702 JTF63_RS03420 WP_001221493.1 715524..716183(-) (ciaR) [Escherichia coli strain 10R]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365