Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   I6K13_RS18500 Genome accession   NZ_CP070162
Coordinates   3805307..3805966 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain FDAARGOS_1283     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3800307..3810966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6K13_RS18485 (I6K13_18480) ygiS 3802512..3804119 (-) 1608 Protein_3669 ABC transporter substrate-binding protein -
  I6K13_RS18490 (I6K13_18485) ygiV 3804228..3804710 (-) 483 WP_000183493.1 GyrI-like domain-containing protein -
  I6K13_RS18495 (I6K13_18490) ygiW 3804763..3805155 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  I6K13_RS18500 (I6K13_18495) ciaR 3805307..3805966 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  I6K13_RS18505 (I6K13_18500) qseC 3805963..3807312 (+) 1350 WP_096953115.1 quorum sensing histidine kinase QseC -
  I6K13_RS18510 (I6K13_18505) ygiZ 3807358..3807690 (-) 333 WP_000914696.1 DUF2645 family protein -
  I6K13_RS25695 - 3807697..3808408 (-) 712 Protein_3675 hypothetical protein -
  I6K13_RS25700 - 3808411..3808890 (-) 480 WP_000069056.1 Hcp family type VI secretion system effector -
  I6K13_RS18520 (I6K13_18515) mdaB 3809140..3809721 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  I6K13_RS18525 (I6K13_18520) ygiN 3809752..3810066 (+) 315 WP_000633738.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=473401 I6K13_RS18500 WP_001221493.1 3805307..3805966(+) (ciaR) [Escherichia coli strain FDAARGOS_1283]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=473401 I6K13_RS18500 WP_001221493.1 3805307..3805966(+) (ciaR) [Escherichia coli strain FDAARGOS_1283]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAAGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCTGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365