Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H3L93_RS02810 Genome accession   NZ_CP059569
Coordinates   525639..527738 (+) Length   699 a.a.
NCBI ID   WP_003797183.1    Uniprot ID   -
Organism   Kingella oralis strain DSM 18271     
Function   pseudopilus extrusion and retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 520639..532738
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H3L93_RS02790 (H3L93_02785) pilM 522675..523778 (+) 1104 WP_003797192.1 type IV pilus biogenesis protein PilM -
  H3L93_RS02795 (H3L93_02790) - 523780..524421 (+) 642 WP_003797190.1 PilN domain-containing protein -
  H3L93_RS02800 (H3L93_02795) pilO 524432..525058 (+) 627 WP_003797188.1 type 4a pilus biogenesis protein PilO -
  H3L93_RS02805 (H3L93_02800) - 525085..525603 (+) 519 WP_040558738.1 pilus assembly protein PilP -
  H3L93_RS02810 (H3L93_02805) pilQ 525639..527738 (+) 2100 WP_003797183.1 type IV pilus secretin PilQ Machinery gene
  H3L93_RS02815 (H3L93_02810) - 527782..528327 (+) 546 WP_040558910.1 shikimate kinase -
  H3L93_RS02820 (H3L93_02815) aroB 528332..529402 (+) 1071 WP_169303486.1 3-dehydroquinate synthase -
  H3L93_RS02825 (H3L93_02820) - 529514..529990 (+) 477 WP_003797177.1 ProQ/FINO family protein -
  H3L93_RS02830 (H3L93_02825) - 530091..530426 (-) 336 WP_155803166.1 hypothetical protein -
  H3L93_RS13280 (H3L93_02830) - 530860..531472 (+) 613 Protein_552 GDSL-type esterase/lipase family protein -
  H3L93_RS02840 (H3L93_02835) - 531624..531794 (-) 171 WP_003797172.1 hypothetical protein -
  H3L93_RS02845 (H3L93_02840) - 531801..532334 (-) 534 WP_003797169.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 699 a.a.        Molecular weight: 76219.42 Da        Isoelectric Point: 10.1426

>NTDB_id=470791 H3L93_RS02810 WP_003797183.1 525639..527738(+) (pilQ) [Kingella oralis strain DSM 18271]
MKKNSVKVLSALVAGLLAQVAYAGSITDIKVSVLPNKQRVIKFKFNKDAVEPTGFITSSPARITLDFSNTDLKVKQPTLT
FNDNLLNQIVTAQSEGNTRVLLGLGQEGQYNVQVKGNEVWVQLSEARSSNSAPVSAVPAARSVQTQSAYSSSVPFNIDFH
KGGNNSGVVEFSSNFTGEPQVRSQSDRLVITLKNYPLPAQAQRNLDVTDFSTPVRTISVRRVGNDTQITLRNQGTWKHTI
TGKNGRQSIQITPTKNVTELGIGANKTKQKFTGKRISLDFQNVDVRTVLQILATESGMNIVASDSVQGKMTLSLKDVPWD
QALDLILDARELDMRRVGNIINVAPRAEMLARDKAELTARKELDDLGPLISQSFQLKYKNVEEFKKILRISDTGSSSGSS
NNSILTSRGSALIDPATNTLIVNDVKSVIQKFNALIEELDVPARQVMVEARIVEASDTLSRELGVKFGGVRAGSTSWANN
WGLATERNSRGVRNGSSGVLFEPNINVPVASAVGSIAVVKSFSSVTLGLELSASEAEGRSKTISTPRVLTQDRQEAEIKQ
GYQVPYTTRDSDGSTTTSFKDAVMSLKVLPRITPDNKIIMDVTINKDTPDNTYQSSEGEPSIRTQSVKTQAMIEDGGTLV
VGGVYQEVIQNNVKKVPMLGDLPVLGNLFKTRSRNNSRNELLFFITPRIMGSETSVMRY

Nucleotide


Download         Length: 2100 bp        

>NTDB_id=470791 H3L93_RS02810 WP_003797183.1 525639..527738(+) (pilQ) [Kingella oralis strain DSM 18271]
ATGAAAAAGAATTCAGTAAAAGTTTTATCAGCACTTGTAGCTGGCTTGCTCGCACAAGTTGCTTATGCTGGCAGCATTAC
CGATATTAAAGTATCTGTTTTGCCTAATAAACAGCGTGTCATCAAGTTTAAATTCAATAAAGATGCGGTAGAGCCAACTG
GTTTTATTACTTCATCTCCTGCCCGTATTACGCTAGATTTTTCAAATACAGATTTGAAAGTAAAACAACCTACTTTAACA
TTCAATGATAATCTACTTAATCAAATTGTCACAGCCCAAAGCGAAGGTAACACTCGCGTTTTGTTGGGATTGGGGCAAGA
AGGTCAATACAATGTTCAGGTAAAAGGCAACGAAGTGTGGGTGCAATTGAGCGAAGCGCGTTCATCTAACTCTGCGCCGG
TAAGTGCCGTTCCTGCTGCAAGAAGCGTACAAACACAAAGTGCTTATTCTTCTTCAGTTCCCTTTAATATTGATTTCCAC
AAAGGTGGCAATAATTCTGGCGTAGTTGAATTTTCATCAAACTTCACTGGCGAGCCCCAAGTTCGTTCACAAAGCGATCG
TTTGGTGATTACACTGAAAAACTATCCATTGCCAGCGCAAGCGCAACGCAATTTGGATGTAACAGATTTTTCTACGCCTG
TTCGCACGATTAGCGTTCGCCGTGTGGGCAACGATACGCAAATCACTCTGCGCAACCAAGGCACATGGAAACATACCATC
ACAGGCAAAAATGGTCGCCAAAGCATCCAAATTACTCCAACTAAAAATGTTACTGAATTGGGCATTGGTGCAAATAAAAC
CAAACAAAAATTCACAGGCAAACGCATCTCTCTTGATTTCCAAAATGTTGATGTGCGAACTGTTTTGCAAATTTTGGCAA
CAGAATCAGGCATGAACATTGTGGCTAGCGATAGCGTGCAAGGCAAAATGACACTTTCGTTGAAAGATGTGCCTTGGGAT
CAAGCGTTGGATTTGATTTTGGATGCGCGCGAATTGGATATGCGCCGCGTGGGCAATATCATCAATGTTGCGCCTCGTGC
TGAAATGTTGGCACGCGATAAAGCGGAATTAACTGCGCGCAAAGAATTGGATGATTTGGGTCCGTTGATTTCTCAAAGTT
TCCAATTGAAATATAAAAATGTTGAAGAATTTAAGAAAATTCTGCGCATCTCTGATACTGGAAGCAGCTCGGGTAGCTCC
AATAACTCTATCTTGACTTCTCGTGGTAGCGCGTTAATTGACCCTGCCACCAACACATTGATTGTTAATGATGTGAAATC
TGTTATCCAAAAATTCAATGCGTTGATTGAAGAGTTGGATGTGCCTGCGCGTCAAGTGATGGTGGAAGCACGGATTGTGG
AAGCATCGGATACATTATCTCGTGAATTGGGTGTGAAATTTGGTGGTGTTCGCGCTGGTAGCACTTCGTGGGCTAACAAT
TGGGGGCTGGCTACTGAACGAAATTCTCGTGGTGTTAGAAATGGTAGTAGTGGAGTTTTGTTTGAGCCTAATATTAACGT
TCCCGTTGCATCGGCGGTTGGCTCAATTGCGGTTGTAAAATCATTTAGCTCTGTTACACTTGGCTTAGAACTTTCCGCTT
CCGAAGCCGAAGGTCGCAGCAAAACCATTTCTACACCTCGCGTTTTAACACAAGATCGCCAAGAAGCTGAAATTAAACAA
GGTTATCAAGTGCCTTACACCACGCGTGATAGCGATGGCAGCACCACAACCTCGTTTAAAGATGCGGTGATGAGCTTGAA
AGTGTTGCCACGTATTACACCTGATAACAAGATTATTATGGATGTTACCATTAACAAAGATACGCCTGATAACACTTACC
AAAGCTCAGAAGGCGAGCCAAGCATTCGTACACAATCGGTGAAAACACAAGCCATGATTGAAGATGGTGGCACTTTGGTT
GTGGGCGGTGTGTATCAAGAAGTGATTCAAAATAACGTGAAAAAAGTGCCTATGCTGGGTGATTTGCCTGTGTTGGGTAA
CTTGTTTAAAACACGTTCGCGCAACAATAGCCGCAATGAATTGCTGTTCTTTATTACGCCTCGCATTATGGGCAGCGAAA
CCAGCGTGATGCGCTACTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(372-445)

Secretin and TonB N terminus short domain (STN)

(299-343)

Bacterial type II and III secretion system protein (Secretin)

(534-690)

AMIN domain (AMIN)

(39-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria meningitidis MC58

47.172

100

0.525
  pilQ Neisseria meningitidis M1080

47.532

100

0.524
  pilQ Neisseria gonorrhoeae strain FA1090

49.727

100

0.521